Mouse Anti-PPP4C Recombinant Antibody (
4B7) (V2LY-0725-LY586)

Name: Mouse Anti-PPP4C Recombinant Antibody (4B7)
SKU: V2LY-0725-LY586
Rating: 5 (1 reviews)

See all PPP4C antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4B7

Application

ELISA, WB, IHC, ICC

Immunogen

Recombinant full length protein (aa 307) of Human PPP4C.

Host Species

Mouse

Specificity

Human, Dog, Mouse, Cattle, Rat

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:500
ICC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Tris-Glycine, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Protein Phosphatase 4 Catalytic Subunit

Entrez Gene ID

5531

UniProt ID

P60510

Function

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on Ser-140 (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity).
In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.

Biological Process

Double-strand break repair via homologous recombinationManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of histone phosphorylationManual Assertion Based On ExperimentIMP:ComplexPortal
Protein dephosphorylationIEA:UniProt
Regulation of double-strand break repairManual Assertion Based On ExperimentIMP:ComplexPortal
Regulation of double-strand break repair via homologous recombinationManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cytoplasm
Nucleus
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

PTM

Methylation at the C-terminal Leu-307 is critical for interactions with regulatory subunits and functions in DNA repair.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-PPP4C Recombinant Antibody (
4B7)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-PPP4C Recombinant Antibody (CB02A) (CAT#: CBMAB-CA002LY)

Mouse Anti-PPP4C Recombinant Antibody (CBYC-P580) (CAT#: CBMAB-P2659-YC)

Mouse Anti-PPP4C Recombinant Antibody (CBT1055) (CAT#: V2LY-0625-LY3073)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control