Rabbit Anti-PRKAR2A Recombinant Antibody (
15G8) (V2LY-0725-LY1032)

Name: Rabbit Anti-PRKAR2A Recombinant Antibody (15G8)
SKU: V2LY-0725-LY1032
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-PRKAR2A Recombinant Antibody (15G8) (V2LY-0725-LY1032) in IHC

IHC image of V2LY-0725-LY1032 diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.47% DAB.

Rabbit Anti-PRKAR2A Recombinant Antibody (15G8) (V2LY-0725-LY1032) in IHC

IHC image of V2LY-0725-LY1032 diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.47% DAB.

Rabbit Anti-PRKAR2A Recombinant Antibody (15G8) (V2LY-0725-LY1032) in IF

Immunofluorescence staining of Hela with V2LY-0725-LY1032 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 512-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-PRKAR2A Recombinant Antibody (15G8) (V2LY-0725-LY1032) in FC

Overlay Peak curve showing MCF-7 cells stained with V2LY-0725-LY1032 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

15G8

Application

ELISA, IHC, IF, FC

Immunogen

A synthesized peptide derived from Human PRKAR2A.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

protein kinase cAMP-dependent type II regulatory subunit alpha

Entrez Gene ID

5576

UniProt ID

P13861

Function

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Biological Process

Intracellular signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of cAMP-dependent protein kinase activityManual Assertion Based On ExperimentIDA:BHF-UCL

Cellular Location

Cytoplasm
Cell membrane
Colocalizes with PJA2 in the cytoplasm and the cell membrane.

PTM

Phosphorylated by the activated catalytic chain.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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