Rabbit Anti-RHEB Recombinant Antibody (
7G7) (V2LY-0725-LY992)

Name: Rabbit Anti-RHEB Recombinant Antibody (7G7)
SKU: V2LY-0725-LY992
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-RHEB Recombinant Antibody (7G7) (V2LY-0725-LY992) in IF

Immunofluorescence staining of Hela Cells with V2LY-0725-LY992 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

7G7

Application

ELISA, IF

Immunogen

A synthesized peptide derived from human RHEB.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IF	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Ras Homolog, MTORC1 Binding

Entrez Gene ID

6009

UniProt ID

Q15382

Function

Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity.

Biological Process

Biological Process negative regulation of cold-induced thermogenesisBy SimilarityISS:YuBioLab
Biological Process oligodendrocyte differentiationIEA:Ensembl
Biological Process positive regulation of oligodendrocyte differentiationIEA:Ensembl
Biological Process positive regulation of TOR signalingManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of cell cycleTAS:Reactome
Biological Process regulation of macroautophagyTAS:Reactome
Biological Process regulation of type B pancreatic cell developmentManual Assertion Based On ExperimentIMP:CACAO
Biological Process signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Biological Process small GTPase mediated signal transductionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Endomembrane system
Golgi apparatus membrane
Cytoplasm, cytosol
Endoplasmic reticulum membrane

PTM

Farnesylation is important for efficiently activating mTORC1-mediated signaling.
Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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