Human Recombinant RHEB protein, His Tag-1 (V2LY-0526-LY6535)

Name: Human Recombinant RHEB protein, His Tag-1
SKU: V2LY-0526-LY6535
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant RHEB protein, His Tag consist of Amino Acid: 1-181 and predicts a molecular mass of 22.4 kDa.

Molecule Mass

22.4 kDa

Sequence

Amino Acid: 1-181

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Ras Homolog, MTORC1 Binding

Function

Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity.

Biological Process

Biological Process negative regulation of cold-induced thermogenesisBy SimilarityISS:YuBioLab
Biological Process oligodendrocyte differentiationIEA:Ensembl
Biological Process positive regulation of oligodendrocyte differentiationIEA:Ensembl
Biological Process positive regulation of TOR signalingManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of cell cycleTAS:Reactome
Biological Process regulation of macroautophagyTAS:Reactome
Biological Process regulation of type B pancreatic cell developmentManual Assertion Based On ExperimentIMP:CACAO
Biological Process signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Biological Process small GTPase mediated signal transductionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Endomembrane system
Golgi apparatus membrane
Cytoplasm, cytosol
Endoplasmic reticulum membrane

PTM

Farnesylation is important for efficiently activating mTORC1-mediated signaling.
Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant RHEB protein, His Tag (CAT#: V2LY-0526-LY6534)

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Human Recombinant RHEB protein, His Tag (CAT#: V2LY-0526-LY6534)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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