Mouse Anti-MSL2 Recombinant Antibody (3A4) (CBMAB-A7662-LY)

Name: Mouse Anti-MSL2 Recombinant Antibody (3A4)
SKU: CBMAB-A7662-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3A4

Application

sELISA, ELISA

Immunogen

RNF184 (NP_060603, 478 a.a. ~ 575 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

MSL Complex Subunit 2

Entrez Gene ID

55167

UniProt ID

Q9HCI7

Alternative Names

FLJ10546; KIAA1585; MSL-2; MSL2L1; RNF184

Function

Component of histone acetyltransferase complex responsible for the majority of histone H4 acetylation at lysine 16 which is implicated in the formation of higher-order chromatin structure. Acts as an E3 ubiquitin ligase that promotes monoubiquitination of histone H2B at 'Lys-35' (H2BK34Ub), but not that of H2A. This activity is greatly enhanced by heterodimerization with MSL1. H2B ubiquitination in turn stimulates histone H3 methylation at 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) and leads to gene activation, including that of HOXA9 and MEIS1.

Biological Process

Histone H4-K16 acetylation Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: Ensembl
Protein ubiquitination Source: GO_Central
Regulation of dosage compensation by inactivation of X chromosome Source: Ensembl

Cellular Location

Nucleus
MSL complex
nucleoplasm
nucleus

Sun, Y., Wiese, M., Hmadi, R., Karayol, R., Seyfferth, J., Martinez Greene, J. A., ... & Akhtar, A. (2023). MSL2 ensures biallelic gene expression in mammals. Nature, 1-9.

Eggers, N., Gkountromichos, F., Krause, S., Campos-Sparr, A., & Becker, P. B. (2023). Physical interaction between MSL2 and CLAMP assures direct cooperativity and prevents competition at composite binding sites. Nucleic Acids Research, 51(17), 9039-9054.

Valsecchi, C. I. K., Basilicata, M. F., Georgiev, P., Gaub, A., Seyfferth, J., Kulkarni, T., ... & Akhtar, A. (2021). RNA nucleation by MSL2 induces selective X chromosome compartmentalization. Nature, 589(7840), 137-142.

Krajewski, W. A., & Vassiliev, O. L. (2019). Analysis of histone ubiquitylation by MSL1/MSL2 proteins in vitro. Archives of biochemistry and biophysics, 666, 22-30.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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