Mouse Anti-RSPO1 Recombinant Antibody (11A9) (CBMAB-R3736-CN)

Name: Mouse Anti-RSPO1 Recombinant Antibody (11A9)
SKU: CBMAB-R3736-CN
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

11A9

Application

WB, IHC

Immunogen

Human recombinant protein fragment corresponding to aa. 21-263 of human RSPO1 (NP_001033722) produced in E.coli

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

More Infomation

Target

Full Name

R-Spondin 1

Introduction

This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Entrez Gene ID

284654

UniProt ID

Q2MKA7

Alternative Names

R-Spondin 1; Roof Plate-Specific Spondin-1; R-Spondin Homolog (Xenopus Laevis); R-Spondin Homolog; R-Spondin-1; CRISTIN3; HRspo1; RSPO;

Function

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors (PubMed:29769720).
Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1 (PubMed:17804805).

Biological Process

Biological Process positive regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of Wnt signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of receptor internalizationManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process Wnt signaling pathwayIEA:UniProtKB-KW

Cellular Location

Secreted
Nucleus
Seems to mainly localize to nucleoli.

Involvement in disease

Keratoderma, palmoplantar, with squamous cell carcinoma of skin and sex reversal (PKKSCC):
A recessive syndrome characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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