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Recombinant Mouse Anti-SIGLEC8 Recombinant Antibody (7C9) (CBMAB-XB1036-YC)

Provided herein is a Mouse Recombinant Antibody against Sialic Acid Binding Ig Like Lectin 8. The antibody can be used for immunoassay techniques, such as ELISA, FC, IP.
See all SIGLEC8 antibodies
Published Data
Fig.1 The fluorescence dot plots show double labeling of cells in the R1, R2, and R3 windows with anti-CD16-phycoerythrin and anti-siglec-8 mAb 7C9 followed by fluorescein isothiocyanate-conjugated anti-mouse IgG.
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Fig.2 Live eosinophil events were identified as DAPI−CCR3+Siglec-F with a purity confirmed by sample back-run.
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Fig.3 Flow-imaging (Imagestreams) micrographs of the surface expression of CAR4 together with eosinophil markers.
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Fig.4 The percentage of eosinophils in bone marrow cultures throughout the 8-day culture was assessed using flow cytometric analysis of surface Siglec-8 expression.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
7C9
Antibody Isotype
IgG1
Application
ELISA, FC, IP

Basic Information

Immunogen
Siglec-8-Fc protein, containing entire extracellular region of Human Siglec-8 fused with the Fc region of human IgG1
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
sialic acid binding Ig-like lectin 8
Introduction
Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs, are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM, and a motif homologous to one identified in signaling lymphocyte activation molecule that mediates an association with SLAM-associated protein.
Entrez Gene ID
27181
UniProt ID
Q9NYZ4
Alternative Names
Sialic Acid Binding Ig Like Lectin 8; Sialoadhesin Family Member 2; SIGLEC-8; SAF-2; SAF2; Sialic Acid Binding Ig-Like Lectin 8; Sialic Acid-Binding Ig-Like Lectin 8; SIGLEC8L; CDw329;
Function
Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells (PubMed:10856141, PubMed:10625619).
Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface (PubMed:10625619).
Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes (PubMed:27357658).
Biological Process
Biological Process cell adhesionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Membrane
Topology
Extracellular: 17-363
Helical: 364-384
Cytoplasmic: 385-499
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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