Human SIGLEC8 ELISA Kit (V2LY-0626-LY5249)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.023 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
54.0 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
sialic acid binding Ig-like lectin 8
Function
Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells (PubMed:10856141, PubMed:10625619).
Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface (PubMed:10625619).
Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes (PubMed:27357658).
Biological Process
Biological Process cell adhesionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Membrane
Topology
Extracellular: 17-363
Helical: 364-384
Cytoplasmic: 385-499
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant SIGLEC8 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY6806)

Human Recombinant SIGLEC8 protein, ECD, His Tag (CAT#: V2LY-0526-LY6807)

Related Products

Human Recombinant SIGLEC8 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY6806)

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Recombinant Mouse Anti-SIGLEC8 Recombinant Antibody (7C9) (CAT#: CBMAB-XB1036-YC)

Mouse Anti-SIGLEC8 Recombinant Antibody (CBXS-1949) (CAT#: CBMAB-S0147-CQ)

Human Anti-SIGLEC8 Recombinant Antibody (CBXS-3572) (CAT#: CBMAB-S0387-CQ)

Mouse Anti-SIGLEC8 Recombinant Antibody (CBXS-0373) (CAT#: CBMAB-S3336-CQ)

Mouse Anti-SIGLEC8 Recombinant Antibody (CBXS-3127) (CAT#: CBMAB-S5870-CQ)

Human Recombinant SIGLEC8 protein, ECD, His Tag (CAT#: V2LY-0526-LY6807)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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