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Mouse Anti-SSH1 Recombinant Antibody (2F9) (CBMAB-A8608-LY)

The product is antibody recognizes SSH1. The antibody 2F9 immunoassay techniques such as: WB, ELISA.
See all SSH1 antibodies
Published Data
Fig.1 Cell cycle-associated changes in the phosphatase activity and gel mobility of SSH1.
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Fig.2 Co-localization of SSH1 with F-actin during mitosis and cytokinesis and aberrant accumulation of F-actin by expression of SSH1(CS).
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Fig.3 An Increase in the P Cofilin Level in ssh Clones and Genetic Interaction between ssh and the Drosophila LIM-Kinase Gene (A–C) Western blot analysis of exogenously expressed Drosophila cofilin/Twinstar (Dcofilin) in S2 cells (A and B) and endogenous one in eye imaginal discs (C).
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Fig.4 Changes in SSH1L protein levels in human pancreatic cancer.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
2F9
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
SSH1 (NP_061857.2, 752 a.a. ~ 849 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Slingshot Protein Phosphatase 1
Introduction
The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).[supplied by OMIM
Entrez Gene ID
54434
UniProt ID
Q8WYL5
Alternative Names
FLJ38102; KIAA1298; SSH-1
Function
Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
Biological Process
Biological Process actin cytoskeleton organizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process cell morphogenesisManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process cellular response to ATPManual Assertion Based On ExperimentIDA:MGI
Biological Process negative regulation of actin filament polymerizationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process protein dephosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of cellular protein metabolic processManual Assertion Based On ExperimentIDA:MGI
Cellular Location
Cytoplasm, cytoskeleton
Cell projection, lamellipodium
Cleavage furrow
Midbody
Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion. Also localized to the cleavage furrow and the midbody during cytokinesis.
PTM
Phosphorylated. Inhibitory phosphorylation by PAK4 promotes binding to YWHAZ. Phosphorylation at Ser-978 is decreased by stimuli which promote actin reorganization and lamellipodia formation. Can be dephosphorylated and activated by PPP3CA/calcineurin A. Phosphorylation decreases immediately prior to telophase.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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