Rabbit Anti-TNFRSF12A Recombinant Antibody (
7H8) (V2LY-0725-LY1213)

Name: Rabbit Anti-TNFRSF12A Recombinant Antibody (7H8)
SKU: V2LY-0725-LY1213
Rating: 5 (1 reviews)

See all TNFRSF12A antibodies

Compare Online Inquiry

Tested Data

Rabbit Anti-TNFRSF12A Recombinant Antibody (7H8) (V2LY-0725-LY1213) in WB

Western Blot
Positive WB detected in: U251 whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate
All lanes: TNFRSF12A antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14, 11 kD
Observed band size: 14 kDa

Rabbit Anti-TNFRSF12A Recombinant Antibody (7H8) (V2LY-0725-LY1213) in IHC

IHC image of V2LY-0725-LY1213 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

7H8

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human TWEAKR.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

tumor necrosis factor receptor superfamily, member 12A

Entrez Gene ID

51330

Function

Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.

Biological Process

Biological Process angiogenesis Source:UniProtKB-KW
Biological Process apoptotic process Source:UniProtKB-KW
Biological Process cell adhesion Source:UniProtKB-KW
Biological Process cell differentiation Source:UniProtKB-KW
Biological Process positive regulation of apoptotic process Source:UniProtKB1 Publication
Biological Process positive regulation of extrinsic apoptotic signaling pathway Source:UniProtKB1 Publication
Biological Process regulation of wound healing Source:UniProtKB1 Publication

Cellular Location

Membrane

Topology

Extracellular: 28-80
Helical: 81-101
Cytoplasmic: 102-129

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-TNFRSF12A Recombinant Antibody (
7H8)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-TNFRSF12A Recombinant Antibody (CBYJT-3704) (CAT#: CBMAB-T3082-YJ)

Mouse Anti-TNFRSF12A Recombinant Antibody (CBXF-2430) (CAT#: CBMAB-F2646-CQ)

Rat Anti-Tnfrsf12a Recombinant Antibody (CBYJT-3712) (CAT#: CBMAB-T3092-YJ)

Rat Anti-TNFRSF12A Recombinant Antibody (CBYY-C1997) (CAT#: CBMAB-C3435-YY)

Mouse Anti-TNFRSF12A Recombinant Antibody (ITEM-4) (CAT#: CBMAB-T3086-YJ)

Mouse Anti-TNFRSF12A Recombinant Antibody (CBYJT-3708) (CAT#: CBMAB-T3088-YJ)

Rat Anti-TNFRSF12A Recombinant Antibody (CBYJT-3705) (CAT#: CBMAB-T3083-YJ)

Rat Anti-TNFRSF12A Recombinant Antibody (314636) (CAT#: CBMAB-C5481-LY)

Rat Anti-Tnfrsf12a Recombinant Antibody (CBYJT-3710) (CAT#: CBMAB-T3090-YJ)

Rat Anti-Tnfrsf12a Recombinant Antibody (CBYJT-3711) (CAT#: CBMAB-T3091-YJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry