Rabbit Anti-TPBG Recombinant Antibody (
6E9) (V2LY-0725-LY1448)

Name: Rabbit Anti-TPBG Recombinant Antibody (6E9)
SKU: V2LY-0725-LY1448
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-TPBG Recombinant Antibody (6E9) (V2LY-0725-LY1448) in WB

Western Blot
Positive WB detected in: U-87 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate
All lanes: 5T4 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 50, 80 kDa

Rabbit Anti-TPBG Recombinant Antibody (6E9) (V2LY-0725-LY1448) in IHC

IHC image of V2LY-0725-LY1448 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

6E9

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human 5T4.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

TPBG Gene(Protein Coding) Trophoblast Glycoprotein

Entrez Gene ID

7162

UniProt ID

Q13641

Function

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.

Biological Process

Biological Process cell adhesion Source:ProtInc1 Publication
Biological Process cell chemotaxis Source:Ensembl
Biological Process dendrite arborization Source:Ensembl
Biological Process mesenchymal cell migration Source:Ensembl
Biological Process negative regulation of canonical Wnt signaling pathway Source:GO_Central1 Publication
Biological Process negative regulation of cell population proliferation Source:Ensembl
Biological Process olfactory learning Source:Ensembl
Biological Process positive regulation of chemotaxis Source:Ensembl
Biological Process positive regulation of ERK1 and ERK2 cascade Source:Ensembl
Biological Process positive regulation of protein kinase B signaling Source:Ensembl
Biological Process positive regulation of synapse assembly Source:Ensembl
Biological Process protein localization to plasma membrane Source:Ensembl
Biological Process synaptic transmission, GABAergic Source:Ensembl

Cellular Location

Cell membrane

Topology

Extracellular: 32-355
Helical: 356-376
Cytoplasmic: 377-420

PTM

Highly glycosylated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

TPBG Matched Antibody Pair (1262) (CAT#: APMAB-1262LY)

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Recombinant Mouse Anti-TPBG Recombinant Antibody (B5C9) (CAT#: CBMAB-XB1177-YC)

Mouse Anti-TPBG (AA 219-328) Recombinant Antibody (CBXO-0454) (CAT#: CBMAB-O0434-CQ)

Mouse Anti-TPBG (AA 31-355) Recombinant Antibody (CBXO-0294) (CAT#: CBMAB-O0435-CQ)

Mouse Anti-TPBG Recombinant Antibody (CBYJT-4299) (CAT#: CBMAB-T3779-YJ)

TPBG Matched Antibody Pair (1262) (CAT#: APMAB-1262LY)

Mouse Anti-TPBG Recombinant Antibody (NDOG1) (CAT#: CBMAB-T3783-YJ)

Recombinant Mouse Anti-TPBG Recombinant Antibody (B1C3) (CAT#: CBMAB-XB1174-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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