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Mouse Anti-TRIM25 Recombinant Antibody (5C3) (CBMAB-A9507-LY)

The product is antibody recognizes TRIM25. The antibody 5C3 immunoassay techniques such as: WB, ELISA.
See all TRIM25 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
5C3
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
TRIM25 (NP_005073, 211 a.a. ~ 310 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
TRIM25 Gene(Protein Coding) Tripartite Motif Containing 25
Introduction
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq]
Entrez Gene ID
7706
UniProt ID
Q14258
Alternative Names
EFP; RNF147; Z147; ZNF147
Function
Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase (PubMed:16352599).
Involved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1 (PubMed:17392790, PubMed:30193849, PubMed:33849980).
Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection (PubMed:17392790, PubMed:23950712).
Mediates 'Lys-63'-linked polyubiquitination of IFIH1 (PubMed:30193849).
Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway (PubMed:16352599, PubMed:17069755).
Mediates estrogen action in various target organs (PubMed:22452784).
Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784).
Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks (By similarity).
Biological Process
Biological Process cellular response to leukemia inhibitory factor Source:Ensembl
Biological Process innate immune response Source:UniProtKB1 Publication
Biological Process negative regulation of viral entry into host cell Source:Ensembl
Biological Process positive regulation of DNA-binding transcription factor activity Source:UniProtKB1 Publication
Biological Process positive regulation of I-kappaB kinase/NF-kappaB signaling Source:UniProtKB1 Publication
Biological Process positive regulation of NF-kappaB transcription factor activity Source:UniProtKB1 Publication
Biological Process protein monoubiquitination Source:ParkinsonsUK-UCL1 Publication
Biological Process regulation of protein localization Source:UniProtKB
Biological Process regulation of viral entry into host cell Source:UniProtKB1 Publication
Biological Process response to estrogen Source:UniProtKB1 Publication
Biological Process response to vitamin D Source:Ensembl
Biological Process suppression of viral release by host Source:Ensembl
Biological Process ubiquitin-dependent ERAD pathway Source:ParkinsonsUK-UCL1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:UniProtKB1 Publication
Biological Process viral release from host cell Source:UniProtKB1 Publication
Cellular Location
Cytoplasm
Cytoplasm, Stress granule
Nucleus
PTM
Auto-ISGylated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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