Mouse Anti-TRIM25 Recombinant Antibody (CBT4286) (V2LY-0625-LY526)
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Basic Information
| Application | Note |
| WB | 1:500-1:2,000 |
| IHC-P | 1:200-1:1,000 |
| ICC | 1:50-1:250 |
| FC | 1:200-1:400 |
| ELISA | 1:10,000 |
Formulations & Storage [For reference only, actual COA shall prevail!]
Target
Involved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1 (PubMed:17392790, PubMed:30193849, PubMed:33849980).
Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection (PubMed:17392790, PubMed:23950712).
Mediates 'Lys-63'-linked polyubiquitination of IFIH1 (PubMed:30193849).
Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway (PubMed:16352599, PubMed:17069755).
Mediates estrogen action in various target organs (PubMed:22452784).
Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784).
Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks (By similarity).
Biological Process innate immune response Source:UniProtKB1 Publication
Biological Process negative regulation of viral entry into host cell Source:Ensembl
Biological Process positive regulation of DNA-binding transcription factor activity Source:UniProtKB1 Publication
Biological Process positive regulation of I-kappaB kinase/NF-kappaB signaling Source:UniProtKB1 Publication
Biological Process positive regulation of NF-kappaB transcription factor activity Source:UniProtKB1 Publication
Biological Process protein monoubiquitination Source:ParkinsonsUK-UCL1 Publication
Biological Process regulation of protein localization Source:UniProtKB
Biological Process regulation of viral entry into host cell Source:UniProtKB1 Publication
Biological Process response to estrogen Source:UniProtKB1 Publication
Biological Process response to vitamin D Source:Ensembl
Biological Process suppression of viral release by host Source:Ensembl
Biological Process ubiquitin-dependent ERAD pathway Source:ParkinsonsUK-UCL1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:UniProtKB1 Publication
Biological Process viral release from host cell Source:UniProtKB1 Publication
Cytoplasm, Stress granule
Nucleus
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols
Antibody Pairs
TRIM25 Matched Antibody Pair (1276) (CAT#: APMAB-1276LY)
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Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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