Rabbit Anti-USP14 Recombinant Antibody (
28C4) (V2LY-0725-LY1283)

Name: Rabbit Anti-USP14 Recombinant Antibody (28C4)
SKU: V2LY-0725-LY1283
Rating: 5 (1 reviews)

See all USP14 antibodies

Compare Online Inquiry

Tested Data

Rabbit Anti-USP14 Recombinant Antibody (28C4) (V2LY-0725-LY1283) in WB

Western BlotPositive WB detected in: PC3 whole cell lysate, Ntera-2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, U251 whole cell lysateAll lanes: USP14 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 57, 53, 55 kDaObserved band size: 55-72 kDa

Rabbit Anti-USP14 Recombinant Antibody (28C4) (V2LY-0725-LY1283) in IF

Immunofluorescence staining of PC-3 cell with V2LY-0725-LY1283 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 517-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

28C4

Application

ELISA, WB, IF

Immunogen

A synthesized peptide derived from human USP14.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IF	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Ubiquitin Specific Peptidase 14

Entrez Gene ID

9097

UniProt ID

P54578

Function

Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome (PubMed:18162577, PubMed:28396413).
Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell (PubMed:18162577).
Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (PubMed:19106094).
Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1 (PubMed:19135427).
Indispensable for synaptic development and function at neuromuscular junctions (NMJs) (By similarity).
Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation (PubMed:27666593).

Biological Process

Biological Process chemical synaptic transmission Source:Ensembl
Biological Process innate immune response Source:UniProtKB-KW
Biological Process negative regulation of ER-associated ubiquitin-dependent protein catabolic process Source:ParkinsonsUK-UCL1 Publication
Biological Process positive regulation of NF-kappaB transcription factor activity Source:Reactome
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic process Source:InterPro
Biological Process protein deubiquitination Source:FlyBase1 Publication
Biological Process regulation of chemotaxis Source:UniProtKB1 Publication
Biological Process regulation of proteasomal protein catabolic process Source:UniProtKB1 Publication

Cellular Location

Cytoplasm
Cell membrane

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-USP14 Recombinant Antibody (
28C4)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

USP14 Matched Antibody Pair (1296) (CAT#: APMAB-1296LY)

Related Products

Mouse Anti-USP14 (aa 395-494) Recombinant Antibody (1F8) (CAT#: CBMAB-U0033-CQ)

Mouse Anti-USP14 Antibody (5G6) (CAT#: CBMAB-1769CQ)

Mouse Anti-USP14 Recombinant Antibody (889CT6.2.1) (CAT#: CBMAB-U0110-CQ)

USP14 Matched Antibody Pair (1296) (CAT#: APMAB-1296LY)

Mouse Anti-USP14 (aa 395-494) Recombinant Antibody (6D6) (CAT#: CBMAB-U0105-CQ)

Mouse Anti-USP14 Antibody (9H6) (CAT#: CBMAB-1770CQ)

Rabbit Anti-USP14 Recombinant Antibody (CBXU-158) (CAT#: CBMAB-U0227-CQ)

Rabbit Anti-USP14 Recombinant Antibody (CBXU-025) (CAT#: CBMAB-U0141-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry