Human CADM4 ELISA Kit (V2LY-0626-LY3962)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.01 ng/mL
Detection Range
0.02-6 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
42.8 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Cell Adhesion Molecule 4
Function
Involved in the cell-cell adhesion. Has calcium- and magnesium-independent cell-cell adhesion activity. May have tumor-suppressor activity.
Biological Process
Cell adhesion Source: UniProtKB-KW
Negative regulation of peptidyl-threonine phosphorylation Source: UniProtKB
Negative regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
Negative regulation of protein phosphorylation Source: UniProtKB
Negative regulation of vascular endothelial growth factor receptor signaling pathway Source: UniProtKB
Negative regulation of vascular endothelial growth factor signaling pathway Source: UniProtKB
Regulation of cell motility Source: UniProtKB
Regulation of cell population proliferation Source: UniProtKB
Regulation of protein phosphorylation Source: UniProtKB
Regulation of Rac protein signal transduction Source: UniProtKB
Regulation of wound healing Source: UniProtKB
Cellular Location
Membrane
Topology
Extracellular: 21-324 aa
Helical: 325-345 aa
Cytoplasmic: 346-388 aa
PTM
N-glycosylated.

Luo, F., Zhao, Y., & Liu, J. (2020). Cell adhesion molecule 4 suppresses cell growth and metastasis by inhibiting the Akt signaling pathway in non-small cell lung cancer. The international journal of biochemistry & cell biology, 123, 105750.

Elazar, N., Vainshtein, A., Golan, N., Vijayaragavan, B., Schaeren-Wiemers, N., Eshed-Eisenbach, Y., & Peles, E. (2019). Axoglial adhesion by Cadm4 regulates CNS myelination. Neuron, 101(2), 224-231.

Meng, X., Maurel, P., Lam, I., Heffernan, C., Stiffler, M. A., McBeath, G., & Salzer, J. L. (2019). Necl‐4/Cadm4 recruits Par‐3 to the Schwann cell adaxonal membrane. Glia, 67(5), 884-895.

Saito, M., Goto, A., Abe, N., Saito, K., Maeda, D., Ohtake, T., ... & Takenoshita, S. (2018). Decreased expression of CADM1 and CADM4 are associated with advanced stage breast cancer. Oncology letters, 15(2), 2401-2406.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CADM4 protein, ECD, His Tag (CAT#: V2LY-0526-LY2504)

Human Recombinant CADM4 protein, hFc Tag (CAT#: V2LY-0526-LY2505)

Mouse Recombinant CADM4 protein, His Tag (CAT#: V2LY-0526-LY7899)

Related Products

Mouse Recombinant CADM4 protein, His Tag (CAT#: V2LY-0526-LY7899)

Mouse Anti-CADM4 Recombinant Antibody (CBYY-I0813) (CAT#: CBMAB-I1808-YY)

Rat Anti-CADM4 Recombinant Antibody (404619) (CAT#: CBMAB-C6440-LY)

Human Recombinant CADM4 protein, ECD, His Tag (CAT#: V2LY-0526-LY2504)

Rabbit Anti-CADM4 Recombinant Antibody (EG460) (CAT#: CBMAB-EN520-LY)

Human Recombinant CADM4 protein, hFc Tag (CAT#: V2LY-0526-LY2505)

Rat Anti-CADM4 Recombinant Antibody (CBYY-I1277) (CAT#: CBMAB-I2446-YY)

Rat Anti-CADM4 Recombinant Antibody (CBYY-C0868) (CAT#: CBMAB-C2304-YY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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