Human CBR1 ELISA Kit (V2LY-0626-LY5553)

Name: Human CBR1 ELISA Kit
SKU: V2LY-0626-LY5553
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0093 ng/mL

Detection Range

0.02-4.5 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

30.4 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Carbonyl Reductase 1

Function

NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol (PubMed:18449627, PubMed:15799708, PubMed:17912391, PubMed:7005231).
Can convert prostaglandin E to prostaglandin F2-alpha (By similarity).
Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione (PubMed:18826943, PubMed:17344335).

Biological Process

Cyclooxygenase pathway Source: Reactome
Drug metabolic process Source: UniProtKB
Epithelial cell differentiation Source: UniProtKB
Positive regulation of reactive oxygen species metabolic process Source: UniProtKB
Vitamin K metabolic process Source: UniProtKB

Cellular Location

Cytoplasm

Mao, L., Wang, K., Zhang, P., Ren, S., Sun, J., Yang, M., ... & Sun, B. (2021). Carbonyl Reductase 1 Attenuates Ischemic Brain Injury by Reducing Oxidative Stress and Neuroinflammation. Translational Stroke Research, 1-14.

Bell, R. M., Villalobos, E., Nixon, M., Miguelez-Crespo, A., Murphy, L., Fawkes, A., ... & Morgan, R. A. (2021). Carbonyl reductase 1 amplifies glucocorticoid action in adipose tissue and impairs glucose tolerance in lean mice. Molecular metabolism, 48, 101225.

Yun, M., Choi, A. J., Woo, S. R., Noh, J. K., Sung, J. Y., Lee, J. W., & Eun, Y. G. (2020). Inhibition of Carbonyl Reductase 1 Enhances Metastasis of Head and Neck Squamous Cell Carcinoma through β-catenin-Mediated Epithelial-Mesenchymal Transition. Journal of Cancer, 11(3), 533.

Koczurkiewicz-Adamczyk, P., Piska, K., Gunia-Krzyżak, A., Bucki, A., Jamrozik, M., Lorenc, E., ... & Pękala, E. (2020). Cinnamic acid derivatives as chemosensitising agents against DOX-treated lung cancer cells–Involvement of carbonyl reductase 1. European Journal of Pharmaceutical Sciences, 154, 105511.

Kajimura, T., Sato, S., Murakami, A., Hayashi‑Okada, M., Nakashima, K., Sueoka, K., & Sugino, N. (2019). Overexpression of carbonyl reductase 1 inhibits malignant behaviors and epithelial mesenchymal transition by suppressing TGF‑β signaling in uterine leiomyosarcoma cells. Oncology letters, 18(2), 1503-1512.

Seliger, J. M., Martin, H. J., Maser, E., & Hintzpeter, J. (2019). Potent inhibition of human carbonyl reductase 1 (CBR1) by the prenylated chalconoid xanthohumol and its related prenylflavonoids isoxanthohumol and 8-prenylnaringenin. Chemico-biological interactions, 305, 156-162.

Piska, K., Koczurkiewicz, P., Wnuk, D., Karnas, E., Bucki, A., Wójcik-Pszczoła, K., ... & Pękala, E. (2019). Synergistic anticancer activity of doxorubicin and piperlongumine on DU-145 prostate cancer cells–the involvement of carbonyl reductase 1 inhibition. Chemico-biological interactions, 300, 40-48.

Yun, M., Choi, A. J., Lee, Y. C., Kong, M., Sung, J. Y., Kim, S. S., & Eun, Y. G. (2018). Carbonyl reductase 1 is a new target to improve the effect of radiotherapy on head and neck squamous cell carcinoma. Journal of Experimental & Clinical Cancer Research, 37(1), 1-12.

Yamanouchi, R., Harada, K., Ferdous, T., & Ueyama, Y. (2018). Low carbonyl reductase 1 expression is associated with poor prognosis in patients with oral squamous cell carcinoma. Molecular and clinical oncology, 8(3), 400-406.

Shi, S. M., & Di, L. (2017). The role of carbonyl reductase 1 in drug discovery and development. Expert opinion on drug metabolism & toxicology, 13(8), 859-870.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

CBR1 Matched Antibody Pair (190) (CAT#: APMAB-190LY)

Antibody Pairs

CBR1 Matched Antibody Pair (190) (CAT#: APMAB-190LY)

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Isotype control

For research use only. Not intended for any clinical use.

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