Mouse Adam12 ELISA Kit (V2LY-0626-LY2313)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.024 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
98.5 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
ADAM Metallopeptidase Domain 12
Function
Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity).
Biological Process
Cell adhesion
Extracellular matrix organization
Myoblast fusion
Positive regulation of angiogenesis
Cellular Location
Isoform 1: Cell membrane
Isoform 2: Secreted
Isoform 3: Secreted
Isoform 4: Secreted
Topology
Extracellular: 208-708 aa
Helical: 709-729 aa
Cytoplasmic: 730-909 aa
PTM
The precursor is cleaved by a furin endopeptidase.

Mohamad, A., Hassan, R., Azlan Husin, M. F. J., & Sulong, S. (2021). Aberrant Methylation of Tumour Suppressor Gene ADAM12 in Chronic Lympocytic Leukemia Patients: Application of Methylation Specific-PCR Technique. Asian Pacific Journal of Cancer Prevention: APJCP, 22(1), 85.

Wang, L., Tan, Z., Zhang, Y., Kady Keita, N., Liu, H., & Zhang, Y. (2020). ADAM12 silencing promotes cellular apoptosis by activating autophagy in choriocarcinoma cells. International journal of oncology, 56(5), 1162-1174.

Bernstein, H. G., Keilhoff, G., Dobrowolny, H., Lendeckel, U., & Steiner, J. (2020). From putative brain tumor marker to high cognitive abilities: Emerging roles of a disintegrin and metalloprotease (ADAM) 12 in the brain. Journal of Chemical Neuroanatomy, 101846.

Aguilar Muniz, L. S., Gonzalez Galarza, F. F., Arguello Astorga, R., Prieto Hinojosa, A. I., Hernandez Teran, F., Mendez Hernandez, A., ... & Arellano Pérez Vertti, R. D. (2020). Analyses of the Genetic Polymorphisms rs3740199 and rs1871054 of the ADAM12 Gene and the Alleles at the rs2073508 Loci of the TGFB1 Gene and Their Contribution to Susceptibility to Primary Knee Osteoarthritis. Genetic testing and molecular biomarkers, 24(6), 375-380.

Albrechtsen, R., Wewer Albrechtsen, N. J., Gnosa, S., Schwarz, J., Dyrskjøt, L., & Kveiborg, M. (2019). Identification of ADAM12 as a novel basigin sheddase. International journal of molecular sciences, 20(8), 1957.

Xiong, L., Yan, W., Zubia, E., Zhou, Y., Zhang, Y., Duan, Q., ... & Xu, G. (2018). Quantitative proteomics and biochemical analyses reveal the role of endoplasmin in the regulation of the expression and secretion of A Disintegrin And Metalloproteinase 12. Journal of proteomics, 182, 34-44.

Hu, X., Sun, G., & Wang, W. (2017). Association of ADAM 12 polymorphisms with the risk of knee osteoarthritis: meta-analysis of 5048 cases and 6848 controls. Rheumatology international, 37(10), 1659-1666.

Wu, Z., Xu, X. W., & Zhang, X. W. (2017). The association of ADAM12 polymorphism with osteoarthritis susceptibility: a meta-analysis. Therapeutics and clinical risk management, 13, 821.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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