Human Recombinant ADAM12 protein, His Tag (V2LY-0526-LY1934)

Name: Human Recombinant ADAM12 protein, His Tag
SKU: V2LY-0526-LY1934
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ADAM12 protein, His Tag consist of Amino Acid: 1-513 and predicts a molecular mass of 55.2 kDa.

Molecule Mass

55.2 kDa

Sequence

Amino Acid: 1-513

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>98% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

ADAM Metallopeptidase Domain 12

Function

Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity).

Biological Process

Cell adhesion
Extracellular matrix organization
Myoblast fusion
Positive regulation of angiogenesis

Cellular Location

Isoform 1: Cell membrane
Isoform 2: Secreted
Isoform 3: Secreted
Isoform 4: Secreted

Topology

Extracellular: 208-708 aa
Helical: 709-729 aa
Cytoplasmic: 730-909 aa

PTM

The precursor is cleaved by a furin endopeptidase.

Mohamad, A., Hassan, R., Azlan Husin, M. F. J., & Sulong, S. (2021). Aberrant Methylation of Tumour Suppressor Gene ADAM12 in Chronic Lympocytic Leukemia Patients: Application of Methylation Specific-PCR Technique. Asian Pacific Journal of Cancer Prevention: APJCP, 22(1), 85.

Wang, L., Tan, Z., Zhang, Y., Kady Keita, N., Liu, H., & Zhang, Y. (2020). ADAM12 silencing promotes cellular apoptosis by activating autophagy in choriocarcinoma cells. International journal of oncology, 56(5), 1162-1174.

Bernstein, H. G., Keilhoff, G., Dobrowolny, H., Lendeckel, U., & Steiner, J. (2020). From putative brain tumor marker to high cognitive abilities: Emerging roles of a disintegrin and metalloprotease (ADAM) 12 in the brain. Journal of Chemical Neuroanatomy, 101846.

Aguilar Muniz, L. S., Gonzalez Galarza, F. F., Arguello Astorga, R., Prieto Hinojosa, A. I., Hernandez Teran, F., Mendez Hernandez, A., ... & Arellano Pérez Vertti, R. D. (2020). Analyses of the Genetic Polymorphisms rs3740199 and rs1871054 of the ADAM12 Gene and the Alleles at the rs2073508 Loci of the TGFB1 Gene and Their Contribution to Susceptibility to Primary Knee Osteoarthritis. Genetic testing and molecular biomarkers, 24(6), 375-380.

Albrechtsen, R., Wewer Albrechtsen, N. J., Gnosa, S., Schwarz, J., Dyrskjøt, L., & Kveiborg, M. (2019). Identification of ADAM12 as a novel basigin sheddase. International journal of molecular sciences, 20(8), 1957.

Xiong, L., Yan, W., Zubia, E., Zhou, Y., Zhang, Y., Duan, Q., ... & Xu, G. (2018). Quantitative proteomics and biochemical analyses reveal the role of endoplasmin in the regulation of the expression and secretion of A Disintegrin And Metalloproteinase 12. Journal of proteomics, 182, 34-44.

Hu, X., Sun, G., & Wang, W. (2017). Association of ADAM 12 polymorphisms with the risk of knee osteoarthritis: meta-analysis of 5048 cases and 6848 controls. Rheumatology international, 37(10), 1659-1666.

Wu, Z., Xu, X. W., & Zhang, X. W. (2017). The association of ADAM12 polymorphism with osteoarthritis susceptibility: a meta-analysis. Therapeutics and clinical risk management, 13, 821.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

ADAM12 Matched Antibody Pair (024) (CAT#: APMAB-024LY)

Antibody Pairs

ADAM12 Matched Antibody Pair (024) (CAT#: APMAB-024LY)

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Rabbit Anti-ADAM12 Recombinant Antibody (V2-179754) (CAT#: CBMAB-A1116-YC)

ADAM12 Matched Antibody Pair (024) (CAT#: APMAB-024LY)

Mouse Anti-ADAM12 Recombinant Antibody (V2-179752) (CAT#: CBMAB-A1114-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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