Human CD27 ELISA Kit (V2LY-0626-LY4383)

Name: Human CD27 ELISA Kit
SKU: V2LY-0626-LY4383
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

1.33 ng/mL

Detection Range

3-900 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

29.1 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

CD27 Molecule

Alternative Names

CD27 Molecule

Function

Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Biological Process

Cell surface receptor signaling pathway Source: UniProtKB
Extrinsic apoptotic signaling pathway Source: BHF-UCL
Immunoglobulin mediated immune response Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of T cell apoptotic process Source: Ensembl
Positive regulation of B cell differentiation Source: UniProtKB
Positive regulation of JNK cascade Source: UniProtKB
Positive regulation of NIK/NF-kappaB signaling Source: UniProtKB
Positive regulation of T cell differentiation Source: InterPro
Response to ethanol Source: Ensembl
Tumor necrosis factor-mediated signaling pathway Source: Reactome

Cellular Location

Membrane

Involvement in disease

Lymphoproliferative syndrome 2 (LPFS2): An autosomal recessive immunodeficiency disorder associated with persistent symptomatic EBV viremia, hypogammaglobulinemia, and impaired T-cell-dependent B-cell responses and T-cell dysfunction. The phenotype is highly variable, ranging from asymptomatic borderline-low hypogammaglobulinemia, to a full-blown symptomatic systemic inflammatory response with life-threatening EBV-related complications, including hemophagocytic lymphohistiocytosis, a lymphoproliferative disorder, and malignant lymphoma requiring stem cell transplantation.

Topology

Extracellular: 20-191
Helical: 192-212
Cytoplasmic: 213-260

PTM

Phosphorylated.
O-glycosylated with core 1 or possibly core 8 glycans.

Xu, F., Zhang, H., Si, X., Chen, J., Chen, Y., Cui, X., & Qin, Y. (2021). Assessment of CD27 expression on T-cells as a diagnostic and therapeutic tool for patients with smear-negative pulmonary tuberculosis. BMC immunology, 22(1), 1-8.

Deng, Y., Chatterjee, B., Zens, K., Zdimerova, H., Müller, A., Schuhmachers, P., ... & Münz, C. (2021). CD27 is required for protective lytic EBV antigen–specific CD8+ T-cell expansion. Blood, The Journal of the American Society of Hematology, 137(23), 3225-3236.

Rojas, J. M., Alejo, A., Avia, J. M., Rodríguez-Martín, D., Sánchez, C., Alcamí, A., ... & Martín, V. (2020). Activation of OX40 and CD27 costimulatory signalling in sheep through recombinant ovine ligands. Vaccines, 8(2), 333.

Nevalainen, T., Autio, A., Kummola, L., Salomaa, T., Junttila, I., Jylhä, M., & Hurme, M. (2019). CD27-IgD-B cell memory subset associates with inflammation and frailty in elderly individuals but only in males. Immunity & Ageing, 16(1), 1-9.

Starzer, A. M., & Berghoff, A. S. (2019). New emerging targets in cancer immunotherapy: CD27 (TNFRSF7). ESMO open, 4, e000629.

Centuori, S. M., Gomes, C. J., Kim, S. S., Putnam, C. W., Larsen, B. T., Garland, L. L., ... & Martinez, J. D. (2018). Double-negative (CD27− IgD−) B cells are expanded in NSCLC and inversely correlate with affinity-matured B cell populations. Journal of translational medicine, 16(1), 1-8.

Alaterre, E., Raimbault, S., Goldschmidt, H., Bouhya, S., Requirand, G., Robert, N., ... & Moreaux, J. (2017). CD24, CD27, CD36 and CD302 gene expression for outcome prediction in patients with multiple myeloma. Oncotarget, 8(58), 98931.

Burris, H. A., Infante, J. R., Ansell, S. M., Nemunaitis, J. J., Weiss, G. R., Villalobos, V. M., ... & Carson, W. E. (2017). Safety and activity of varlilumab, a novel and first-in-class agonist anti-CD27 antibody, in patients with advanced solid tumors. J Clin Oncol, 35(18), 2028-2036.

Chen, D., Gerasimčik, N., Camponeschi, A., Tan, Y., Wu, Q., Brynjolfsson, S., ... & Mårtensson, I. L. (2017). CD27 expression and its association with clinical outcome in children and adults with pro-B acute lymphoblastic leukemia. Blood cancer journal, 7(6), e575-e575.

Bullock, T. N. (2017). Stimulating CD27 to quantitatively and qualitatively shape adaptive immunity to cancer. Current opinion in immunology, 45, 82-88.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD27 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2750)

Human Recombinant CD27 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2751)

Human Recombinant CD27 protein, His Tag (CAT#: V2LY-0526-LY2752)

Human Recombinant CD27 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2753)

Human Recombinant CD27 protein, His Tag-1 (CAT#: V2LY-0526-LY2754)

Human Recombinant CD27 protein, rFc Tag (CAT#: V2LY-0526-LY2755)

Mouse Recombinant CD27 protein, ECD, Fc Tag (CAT#: V2LY-0526-LY7981)

Mouse Recombinant CD27 protein, His & hFc Tag (CAT#: V2LY-0526-LY7982)

Mouse Recombinant CD27 protein, His Tag (CAT#: V2LY-0526-LY7983)

Rat Recombinant CD27 protein, hFc Tag (CAT#: V2LY-0526-LY9223)

Antibody Pairs

CD27 Matched Antibody Pair (213) (CAT#: APMAB-213LY)

Related Products

Rat Anti-CD27 Recombinant Antibody (137915) (CAT#: CBMAB-C0305-LY)

Human Recombinant CD27 protein, rFc Tag (CAT#: V2LY-0526-LY2755)

Mouse Anti-CD27 Recombinant Antibody (CBT4261) (CAT#: V2LY-0625-LY1222)

Human Recombinant CD27 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2753)

Mouse Recombinant CD27 protein, ECD, Fc Tag (CAT#: V2LY-0526-LY7981)

Mouse Recombinant CD27 protein, His Tag (CAT#: V2LY-0526-LY7983)

Human Recombinant CD27 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2750)

Rabbit Anti-CD27 Recombinant Antibody (
4A1) (CAT#: V2LY-0725-LY1523)

Human Recombinant CD27 protein, His Tag (CAT#: V2LY-0526-LY2752)

CD27 Matched Antibody Pair (213) (CAT#: APMAB-213LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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