Mouse Recombinant CD27 protein, ECD, Fc Tag (V2LY-0526-LY7981)

Name: Mouse Recombinant CD27 protein, ECD, Fc Tag
SKU: V2LY-0526-LY7981
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

Fc Tag

Protein Construction

This product is Mouse Recombinant CD27 protein, ECD, Fc Tag consist of Amino Acid: 1-182 and predicts a molecular mass of 44.17 kDa.

Molecule Mass

44.17 kDa

Protein Domain

ECD

Sequence

Amino Acid: 1-182

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD27 Molecule

Alternative Names

CD27 Molecule

Function

Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Biological Process

Cell surface receptor signaling pathway Source: UniProtKB
Extrinsic apoptotic signaling pathway Source: BHF-UCL
Immunoglobulin mediated immune response Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of T cell apoptotic process Source: Ensembl
Positive regulation of B cell differentiation Source: UniProtKB
Positive regulation of JNK cascade Source: UniProtKB
Positive regulation of NIK/NF-kappaB signaling Source: UniProtKB
Positive regulation of T cell differentiation Source: InterPro
Response to ethanol Source: Ensembl
Tumor necrosis factor-mediated signaling pathway Source: Reactome

Cellular Location

Membrane

Involvement in disease

Lymphoproliferative syndrome 2 (LPFS2): An autosomal recessive immunodeficiency disorder associated with persistent symptomatic EBV viremia, hypogammaglobulinemia, and impaired T-cell-dependent B-cell responses and T-cell dysfunction. The phenotype is highly variable, ranging from asymptomatic borderline-low hypogammaglobulinemia, to a full-blown symptomatic systemic inflammatory response with life-threatening EBV-related complications, including hemophagocytic lymphohistiocytosis, a lymphoproliferative disorder, and malignant lymphoma requiring stem cell transplantation.

Topology

Extracellular: 20-191
Helical: 192-212
Cytoplasmic: 213-260

PTM

Phosphorylated.
O-glycosylated with core 1 or possibly core 8 glycans.

Xu, F., Zhang, H., Si, X., Chen, J., Chen, Y., Cui, X., & Qin, Y. (2021). Assessment of CD27 expression on T-cells as a diagnostic and therapeutic tool for patients with smear-negative pulmonary tuberculosis. BMC immunology, 22(1), 1-8.

Deng, Y., Chatterjee, B., Zens, K., Zdimerova, H., Müller, A., Schuhmachers, P., ... & Münz, C. (2021). CD27 is required for protective lytic EBV antigen–specific CD8+ T-cell expansion. Blood, The Journal of the American Society of Hematology, 137(23), 3225-3236.

Rojas, J. M., Alejo, A., Avia, J. M., Rodríguez-Martín, D., Sánchez, C., Alcamí, A., ... & Martín, V. (2020). Activation of OX40 and CD27 costimulatory signalling in sheep through recombinant ovine ligands. Vaccines, 8(2), 333.

Nevalainen, T., Autio, A., Kummola, L., Salomaa, T., Junttila, I., Jylhä, M., & Hurme, M. (2019). CD27-IgD-B cell memory subset associates with inflammation and frailty in elderly individuals but only in males. Immunity & Ageing, 16(1), 1-9.

Starzer, A. M., & Berghoff, A. S. (2019). New emerging targets in cancer immunotherapy: CD27 (TNFRSF7). ESMO open, 4, e000629.

Centuori, S. M., Gomes, C. J., Kim, S. S., Putnam, C. W., Larsen, B. T., Garland, L. L., ... & Martinez, J. D. (2018). Double-negative (CD27− IgD−) B cells are expanded in NSCLC and inversely correlate with affinity-matured B cell populations. Journal of translational medicine, 16(1), 1-8.

Alaterre, E., Raimbault, S., Goldschmidt, H., Bouhya, S., Requirand, G., Robert, N., ... & Moreaux, J. (2017). CD24, CD27, CD36 and CD302 gene expression for outcome prediction in patients with multiple myeloma. Oncotarget, 8(58), 98931.

Burris, H. A., Infante, J. R., Ansell, S. M., Nemunaitis, J. J., Weiss, G. R., Villalobos, V. M., ... & Carson, W. E. (2017). Safety and activity of varlilumab, a novel and first-in-class agonist anti-CD27 antibody, in patients with advanced solid tumors. J Clin Oncol, 35(18), 2028-2036.

Chen, D., Gerasimčik, N., Camponeschi, A., Tan, Y., Wu, Q., Brynjolfsson, S., ... & Mårtensson, I. L. (2017). CD27 expression and its association with clinical outcome in children and adults with pro-B acute lymphoblastic leukemia. Blood cancer journal, 7(6), e575-e575.

Bullock, T. N. (2017). Stimulating CD27 to quantitatively and qualitatively shape adaptive immunity to cancer. Current opinion in immunology, 45, 82-88.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD27 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2750)

Human Recombinant CD27 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2751)

Human Recombinant CD27 protein, His Tag (CAT#: V2LY-0526-LY2752)

Human Recombinant CD27 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2753)

Human Recombinant CD27 protein, His Tag-1 (CAT#: V2LY-0526-LY2754)

Human Recombinant CD27 protein, rFc Tag (CAT#: V2LY-0526-LY2755)

Mouse Recombinant CD27 protein, His & hFc Tag (CAT#: V2LY-0526-LY7982)

Mouse Recombinant CD27 protein, His Tag (CAT#: V2LY-0526-LY7983)

Rat Recombinant CD27 protein, hFc Tag (CAT#: V2LY-0526-LY9223)

Rat Recombinant CD27 protein, Fc Tag (CAT#: V2LY-0526-LY9224)

Antibody Pairs

CD27 Matched Antibody Pair (213) (CAT#: APMAB-213LY)

Related Products

Rat Recombinant CD27 protein, hFc Tag (CAT#: V2LY-0526-LY9223)

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Mouse Recombinant CD27 protein, His & hFc Tag (CAT#: V2LY-0526-LY7982)

Human Recombinant CD27 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2750)

Human Recombinant CD27 protein, His Tag-1 (CAT#: V2LY-0526-LY2754)

Rabbit Anti-CD27 Recombinant Antibody (CBFYC-1185) (CAT#: CBMAB-C1243-FY)

Mouse Recombinant CD27 protein, His Tag (CAT#: V2LY-0526-LY7983)

Human Recombinant CD27 protein, His Tag (CAT#: V2LY-0526-LY2752)

Rat Anti-CD27 Recombinant Antibody (137910) (CAT#: CBMAB-C0304-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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