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Mouse Anti-CD27 Recombinant Antibody (CBFYC-1181) (CBMAB-C1239-FY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYC-1181
Antibody Isotype
IgG2a, κ
Application
WB, IP, ELISA, IF

Basic Information

Immunogen
Amino acids 221-249 at the C-terminus of CD27 of mouse origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD27 Molecule
Introduction
CD27 (CD27 Molecule) is a Protein Coding gene. Diseases associated with CD27 include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Disease. Among its related pathways are Cytokine Signaling in Immune system and Akt Signaling. Gene Ontology (GO) annotations related to this gene include transmembrane signaling receptor activity and cysteine-type endopeptidase inhibitor activity involved in apoptotic process.
Entrez Gene ID
939
UniProt ID
P26842
Alternative Names
CD27 Molecule
Function
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Biological Process
Cell surface receptor signaling pathway Source: UniProtKB
Extrinsic apoptotic signaling pathway Source: BHF-UCL
Immunoglobulin mediated immune response Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of T cell apoptotic process Source: Ensembl
Positive regulation of B cell differentiation Source: UniProtKB
Positive regulation of JNK cascade Source: UniProtKB
Positive regulation of NIK/NF-kappaB signaling Source: UniProtKB
Positive regulation of T cell differentiation Source: InterPro
Response to ethanol Source: Ensembl
Tumor necrosis factor-mediated signaling pathway Source: Reactome
Cellular Location
Membrane
Involvement in disease
Lymphoproliferative syndrome 2 (LPFS2): An autosomal recessive immunodeficiency disorder associated with persistent symptomatic EBV viremia, hypogammaglobulinemia, and impaired T-cell-dependent B-cell responses and T-cell dysfunction. The phenotype is highly variable, ranging from asymptomatic borderline-low hypogammaglobulinemia, to a full-blown symptomatic systemic inflammatory response with life-threatening EBV-related complications, including hemophagocytic lymphohistiocytosis, a lymphoproliferative disorder, and malignant lymphoma requiring stem cell transplantation.
Topology
Extracellular: 20-191
Helical: 192-212
Cytoplasmic: 213-260
PTM
Phosphorylated.
O-glycosylated with core 1 or possibly core 8 glycans.
More Infomation

Xu, F., Zhang, H., Si, X., Chen, J., Chen, Y., Cui, X., & Qin, Y. (2021). Assessment of CD27 expression on T-cells as a diagnostic and therapeutic tool for patients with smear-negative pulmonary tuberculosis. BMC immunology, 22(1), 1-8.

Deng, Y., Chatterjee, B., Zens, K., Zdimerova, H., Müller, A., Schuhmachers, P., ... & Münz, C. (2021). CD27 is required for protective lytic EBV antigen–specific CD8+ T-cell expansion. Blood, The Journal of the American Society of Hematology, 137(23), 3225-3236.

Rojas, J. M., Alejo, A., Avia, J. M., Rodríguez-Martín, D., Sánchez, C., Alcamí, A., ... & Martín, V. (2020). Activation of OX40 and CD27 costimulatory signalling in sheep through recombinant ovine ligands. Vaccines, 8(2), 333.

Nevalainen, T., Autio, A., Kummola, L., Salomaa, T., Junttila, I., Jylhä, M., & Hurme, M. (2019). CD27-IgD-B cell memory subset associates with inflammation and frailty in elderly individuals but only in males. Immunity & Ageing, 16(1), 1-9.

Starzer, A. M., & Berghoff, A. S. (2019). New emerging targets in cancer immunotherapy: CD27 (TNFRSF7). ESMO open, 4, e000629.

Centuori, S. M., Gomes, C. J., Kim, S. S., Putnam, C. W., Larsen, B. T., Garland, L. L., ... & Martinez, J. D. (2018). Double-negative (CD27− IgD−) B cells are expanded in NSCLC and inversely correlate with affinity-matured B cell populations. Journal of translational medicine, 16(1), 1-8.

Alaterre, E., Raimbault, S., Goldschmidt, H., Bouhya, S., Requirand, G., Robert, N., ... & Moreaux, J. (2017). CD24, CD27, CD36 and CD302 gene expression for outcome prediction in patients with multiple myeloma. Oncotarget, 8(58), 98931.

Burris, H. A., Infante, J. R., Ansell, S. M., Nemunaitis, J. J., Weiss, G. R., Villalobos, V. M., ... & Carson, W. E. (2017). Safety and activity of varlilumab, a novel and first-in-class agonist anti-CD27 antibody, in patients with advanced solid tumors. J Clin Oncol, 35(18), 2028-2036.

Chen, D., Gerasimčik, N., Camponeschi, A., Tan, Y., Wu, Q., Brynjolfsson, S., ... & Mårtensson, I. L. (2017). CD27 expression and its association with clinical outcome in children and adults with pro-B acute lymphoblastic leukemia. Blood cancer journal, 7(6), e575-e575.

Bullock, T. N. (2017). Stimulating CD27 to quantitatively and qualitatively shape adaptive immunity to cancer. Current opinion in immunology, 45, 82-88.

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For research use only. Not intended for any clinical use.

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