Human CD28 ELISA Kit (V2LY-0626-LY4099)

Name: Human CD28 ELISA Kit
SKU: V2LY-0626-LY4099
Rating: 5 (1 reviews)

See all CD28 antibodies

Compare Online Inquiry

Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.024 ng/mL

Detection Range

0.05-30 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

25.1 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

CD28 Molecule

Alternative Names

CD28 Molecule; CD28 Antigen; Tp44; T-Cell-Specific Surface Glycoprotein CD28; T-Cell-Specific Surface Glycoprotein; CD28 Antigen (Tp44);

Function

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933).
Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).

Biological Process

Apoptotic signaling pathway Source: Ensembl
Cell surface receptor signaling pathway Source: UniProtKB
Humoral immune response Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of gene expression Source: UniProtKB
Negative thymic T cell selection Source: Ensembl
Positive regulation of alpha-beta T cell proliferation Source: Ensembl
Positive regulation of cytokine production Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of inflammatory response to antigenic stimulus Source: Ensembl
Positive regulation of interleukin-10 production Source: UniProtKB
Positive regulation of interleukin-2 production Source: UniProtKB
Positive regulation of interleukin-4 production Source: UniProtKB
Positive regulation of isotype switching to IgG isotypes Source: Ensembl
Positive regulation of mitotic nuclear division Source: UniProtKB
Positive regulation of phosphatidylinositol 3-kinase signaling Source: Ensembl
Positive regulation of protein kinase B signaling Source: Reactome
Positive regulation of T cell proliferation Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of translation Source: UniProtKB
Positive regulation of viral genome replication Source: UniProtKB
Regulation of defense response to virus by virus Source: Reactome
Regulation of regulatory T cell differentiation Source: Ensembl
Regulatory T cell differentiation Source: BHF-UCL
T cell activation Source: BHF-UCL
T cell costimulation Source: GO_Central
T cell receptor signaling pathway Source: GO_Central

Cellular Location

Membrane
Isoform 3: Cell surface

Topology

Extracellular: 19-152
Helical: 153-179
Cytoplasmic: 180-220

PTM

CD40LG induces tyrosine phosphorylation of isoform 3.

Matundan, H. H., Jaggi, U., Yu, J., Akbari, O., & Ghiasi, H. (2021). Absence of CD28-CTLA4-PD-L1 Costimulatory Molecules Reduces Herpes Simplex Virus 1 Reactivation. Mbio, 12(4), e01176-21.

Nagai, S., & Azuma, M. (2019). The CD28–B7 family of co-signaling molecules. Co-signal Molecules in T Cell Activation, 25-51.

Huff, W. X., Kwon, J. H., Henriquez, M., Fetcko, K., & Dey, M. (2019). The evolving role of CD8+ CD28− immunosenescent T cells in cancer immunology. International journal of molecular sciences, 20(11), 2810.

Hosoe, Y., Numoto, N., Inaba, S., Ogawa, S., Morii, H., Abe, R., ... & Oda, M. (2019). Structural and functional properties of Grb2 SH2 dimer in CD28 binding. Biophysics and physicobiology, 16, 80-88.

Siokis, A., Robert, P. A., Demetriou, P., Dustin, M. L., & Meyer-Hermann, M. (2018). F-actin-driven CD28-CD80 localization in the immune synapse. Cell reports, 24(5), 1151-1162.

Huang, Y., Wang, Z., Zheng, Q., Tang, J., Cai, J., Lu, Y., & Jian, J. (2018). Conservation of structural and interactional features of CD28 and CD80/86 molecules from Nile tilapia (Oreochromis niloticus). Fish & shellfish immunology, 72, 95-103.

Xia, F., Qian, C. R., Xun, Z., Hamon, Y., Sartre, A. M., Formisano, A., ... & He, H. T. (2018). TCR and CD28 concomitant stimulation elicits a distinctive calcium response in naive T cells. Frontiers in immunology, 9, 2864.

Porciello, N., Grazioli, P., Campese, A. F., Kunkl, M., Caristi, S., Mastrogiovanni, M., ... & Tuosto, L. (2018). A non-conserved amino acid variant regulates differential signalling between human and mouse CD28. Nature communications, 9(1), 1-16.

Yang, W., Pan, W., Chen, S., Trendel, N., Jiang, S., Xiao, F., ... & Xu, C. (2017). Dynamic regulation of CD28 conformation and signaling by charged lipids and ions. Nature structural & molecular biology, 24(12), 1081-1092.

Zumerle, S., Molon, B., & Viola, A. (2017). Membrane rafts in T cell activation: a spotlight on CD28 costimulation. Frontiers in immunology, 8, 1467.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human CD28 ELISA Kit?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

CD28 Matched Antibody Pair (216) (CAT#: APMAB-216LY)

Cynomolgus Recombinant CD28 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY1423)

Human Recombinant CD28 protein, Dimer, ECD, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2760)

Human Recombinant CD28 protein, Dimer, ECD, hFc Tag (CAT#: V2LY-0526-LY2761)

Human Recombinant CD28 protein, AF488 Conjugated, His Tag (CAT#: V2LY-0526-LY2762)

Human Recombinant CD28 protein, AF647 Conjugated, His Tag (CAT#: V2LY-0526-LY2763)

Human Recombinant CD28 protein, APC Conjugated, His Tag (CAT#: V2LY-0526-LY2764)

Human Recombinant CD28 protein, PE Conjugated, His Tag (CAT#: V2LY-0526-LY2765)

Human, Cynomolgus, Rhesus Recombinant CD28 protein, Dimer, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY7703)

Human, Cynomolgus, Rhesus Recombinant CD28 protein, Dimer, ECD, Fc Tag (CAT#: V2LY-0526-LY7704)

Antibody Pairs

CD28 Matched Antibody Pair (216) (CAT#: APMAB-216LY)

Related Products

Mouse Recombinant CD28 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY7986)

Mouse Anti-CD28 Recombinant Antibody (CBT3479) (CAT#: V2LY-0625-LY2021)

Syrian Hamster Anti-CD28 Recombinant Antibody (37.51) (CAT#: CBMAB-0978-LY)

Mouse Recombinant CD28 protein, Dimer, ECD, hFc Tag (CAT#: V2LY-0526-LY7984)

Rat Anti-CD28 Recombinant Antibody (61109) (CAT#: CBMAB-C7870-LY)

Cynomolgus Recombinant CD28 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY1423)

Human Recombinant CD28 protein, AF647 Conjugated, His Tag (CAT#: V2LY-0526-LY2763)

Rat Cd28 ELISA Kit (CAT#: V2LY-0626-LY2710)

Mouse Anti-CD28 Recombinant Antibody (9.3) (CAT#: CBMAB-0920-LY)

Mouse Anti-CD28 Recombinant Antibody (CBFYC-1208) (CAT#: CBMAB-C1266-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry