Human CD58 ELISA Kit (V2LY-0626-LY562)

Name: Human CD58 ELISA Kit
SKU: V2LY-0626-LY562
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.024 ng/mL

Detection Range

0.05-20 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

28.1 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

CD58 Molecule

Function

Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.

Biological Process

Cell-cell adhesion Source: UniProtKB
Cellular response to interferon-gamma Source: UniProtKB
Cellular response to tumor necrosis factor Source: UniProtKB
Heterotypic cell-cell adhesion Source: UniProtKB
Leukocyte migration Source: Reactome
Neutrophil degranulation Source: Reactome
Positive regulation of interleukin-8 production Source: UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 29-215
Helical: 216-238
Cytoplasmic: 239-250

Zhang, Y., Liu, Q., Yang, S., & Liao, Q. (2021). CD58 Immunobiology at a Glance. Frontiers in Immunology, 12, 2212.

Zhang, Y., Liu, Q., Liu, J., & Liao, Q. (2021). Upregulated CD58 is associated with clinicopathological characteristics and poor prognosis of patients with pancreatic ductal adenocarcinoma. Cancer Cell International, 21(1), 1-15.

Tripathi, N., Leherte, L., Vercauteren, D. P., & Laurent, A. D. (2021). Structure-based identification of inhibitors disrupting the CD2–CD58 interactions. Journal of computer-aided molecular design, 35(3), 337-353.

Parajuli, P., Sable, R., Shrestha, L., Dahal, A., Gauthier, T., Taneja, V., & Jois, S. (2021). Modulation of co‐stimulatory signal from CD2–CD58 proteins by a grafted peptide. Chemical Biology & Drug Design, 97(3), 607-627.

Otsuka, Y., Nishikori, M., Arima, H., Izumi, K., Kitawaki, T., Hishizawa, M., & Takaori-Kondo, A. (2020). EZH2 inhibitors restore epigenetically silenced CD58 expression in B-cell lymphomas. Molecular immunology, 119, 35-45.

Yamamoto, M., Watanabe, M., Inoue, N., Watanabe, A., Ozaki, H., Ohsaki, M., ... & Iwatani, Y. (2020). Association of CD58 polymorphisms and its protein expression with the development and prognosis of autoimmune thyroid diseases. Immunological investigations, 49(1-2), 106-119.

Veldman, J., Visser, L., Huberts-Kregel, M., Muller, N., Hepkema, B., Van den Berg, A., & Diepstra, A. (2020). Rosetting T cells in Hodgkin lymphoma are activated by immunological synapse components HLA class II and CD58. Blood, 136(21), 2437-2441.

Leherte, L., Petit, A., Jacquemin, D., Vercauteren, D. P., & Laurent, A. D. (2018). Investigating cyclic peptides inhibiting CD2–CD58 interactions through molecular dynamics and molecular docking methods. Journal of computer-aided molecular design, 32(11), 1295-1313.

Lu, Y. J., Qiu, L. L., & Wang, L. L. (2017). Research Progress on Relationship between CD58 Molecule and ALL and Lymphoma-Review. Zhongguo shi yan xue ye xue za zhi, 25(2), 592-595.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant CD58 protein, His Tag (CAT#: V2LY-0526-LY1445)

Human Recombinant CD58 protein, hFc Tag (CAT#: V2LY-0526-LY2904)

Human Recombinant CD58 protein, His Tag (CAT#: V2LY-0526-LY2905)

CD58 Matched Antibody Pair (231) (CAT#: APMAB-231LY)

Antibody Pairs

CD58 Matched Antibody Pair (231) (CAT#: APMAB-231LY)

Related Products

Cynomolgus Recombinant CD58 protein, His Tag (CAT#: V2LY-0526-LY1445)

Human Recombinant CD58 protein, His Tag (CAT#: V2LY-0526-LY2905)

Rabbit Anti-CD58 Recombinant Antibody (CBYY-C0267) (CAT#: CBMAB-C0782-YY)

Mouse Anti-CD58 Recombinant Antibody (CBFYC-1369) (CAT#: CBMAB-C1427-FY)

Mouse Anti-CD58 Recombinant Antibody (BRIC-5) (CAT#: CBMAB-C1860-CN)

Mouse Anti-CD58 Recombinant Antibody (CBFYC-0168) (CAT#: CBMAB-C0191-FY)

Rabbit Anti-CD58 Recombinant Antibody (CBYJL-1015) (CAT#: CBMAB-L0425-YJ)

Human Recombinant CD58 protein, hFc Tag (CAT#: V2LY-0526-LY2904)

Rabbit Anti-CD58 Recombinant Antibody (
13G5) (CAT#: V2LY-0725-LY988)

Mouse Anti-CD58 Recombinant Antibody (CBFYC-0381) (CAT#: CBMAB-C0421-FY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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