Human Recombinant CD58 protein, His Tag (V2LY-0526-LY2905)

Name: Human Recombinant CD58 protein, His Tag
SKU: V2LY-0526-LY2905
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CD58 protein, His Tag consist of Amino Acid: 1-215 and predicts a molecular mass of 22.9 kDa.

Molecule Mass

22.9 kDa

Verified

HPLC

Sequence

Amino Acid: 1-215

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD58 Molecule

Function

Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.

Biological Process

Cell-cell adhesion Source: UniProtKB
Cellular response to interferon-gamma Source: UniProtKB
Cellular response to tumor necrosis factor Source: UniProtKB
Heterotypic cell-cell adhesion Source: UniProtKB
Leukocyte migration Source: Reactome
Neutrophil degranulation Source: Reactome
Positive regulation of interleukin-8 production Source: UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 29-215
Helical: 216-238
Cytoplasmic: 239-250

Zhang, Y., Liu, Q., Yang, S., & Liao, Q. (2021). CD58 Immunobiology at a Glance. Frontiers in Immunology, 12, 2212.

Zhang, Y., Liu, Q., Liu, J., & Liao, Q. (2021). Upregulated CD58 is associated with clinicopathological characteristics and poor prognosis of patients with pancreatic ductal adenocarcinoma. Cancer Cell International, 21(1), 1-15.

Tripathi, N., Leherte, L., Vercauteren, D. P., & Laurent, A. D. (2021). Structure-based identification of inhibitors disrupting the CD2–CD58 interactions. Journal of computer-aided molecular design, 35(3), 337-353.

Parajuli, P., Sable, R., Shrestha, L., Dahal, A., Gauthier, T., Taneja, V., & Jois, S. (2021). Modulation of co‐stimulatory signal from CD2–CD58 proteins by a grafted peptide. Chemical Biology & Drug Design, 97(3), 607-627.

Otsuka, Y., Nishikori, M., Arima, H., Izumi, K., Kitawaki, T., Hishizawa, M., & Takaori-Kondo, A. (2020). EZH2 inhibitors restore epigenetically silenced CD58 expression in B-cell lymphomas. Molecular immunology, 119, 35-45.

Yamamoto, M., Watanabe, M., Inoue, N., Watanabe, A., Ozaki, H., Ohsaki, M., ... & Iwatani, Y. (2020). Association of CD58 polymorphisms and its protein expression with the development and prognosis of autoimmune thyroid diseases. Immunological investigations, 49(1-2), 106-119.

Veldman, J., Visser, L., Huberts-Kregel, M., Muller, N., Hepkema, B., Van den Berg, A., & Diepstra, A. (2020). Rosetting T cells in Hodgkin lymphoma are activated by immunological synapse components HLA class II and CD58. Blood, 136(21), 2437-2441.

Leherte, L., Petit, A., Jacquemin, D., Vercauteren, D. P., & Laurent, A. D. (2018). Investigating cyclic peptides inhibiting CD2–CD58 interactions through molecular dynamics and molecular docking methods. Journal of computer-aided molecular design, 32(11), 1295-1313.

Lu, Y. J., Qiu, L. L., & Wang, L. L. (2017). Research Progress on Relationship between CD58 Molecule and ALL and Lymphoma-Review. Zhongguo shi yan xue ye xue za zhi, 25(2), 592-595.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant CD58 protein, His Tag (CAT#: V2LY-0526-LY1445)

Human Recombinant CD58 protein, hFc Tag (CAT#: V2LY-0526-LY2904)

Human CD58 ELISA Kit (CAT#: V2LY-0626-LY562)

CD58 Matched Antibody Pair (231) (CAT#: APMAB-231LY)

Antibody Pairs

CD58 Matched Antibody Pair (231) (CAT#: APMAB-231LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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