Human CD70 ELISA Kit (V2LY-0626-LY4386)

Name: Human CD70 ELISA Kit
SKU: V2LY-0626-LY4386
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.023 ng/mL

Detection Range

0.05-20 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

21.1 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

CD70 Molecule

Alternative Names

CD70 Molecule; Tumor Necrosis Factor Ligand Superfamily Member 7; CD70 Antigen; CD27 Ligand; CD27LG; TNFSF7; CD27-L;

Function

Cytokine which is the ligand for CD27. The CD70-CD27 pathway plays an important role in the generation and maintenance of T cell immunity, in particular during antiviral responses. Upon CD27 binding, induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

Biological Process

B cell mediated immunity Source: UniProtKB
B cell proliferation Source: UniProtKB
Cell-cell signaling Source: ProtInc
Extrinsic apoptotic signaling pathway Source: BHF-UCL
Positive regulation of T cell proliferation Source: GO_Central
Signal transduction Source: ProtInc
T cell mediated immunity Source: UniProtKB
Tumor necrosis factor-mediated signaling pathway Source: Reactome

Cellular Location

Membrane

Involvement in disease

Lymphoproliferative syndrome 3 (LPFS3): An autosomal recessive, early-onset immunologic disorder characterized by increased susceptibility to Epstein-Barr virus infection in B cells, abnormal B-cell proliferation and increased susceptibility to B-cell malignancies, including Hodgkin lymphoma. Patients usually have lymphadenopathy and hypogammaglobulinemia, and may suffer from recurrent infections.

Topology

Cytoplasmic: 1-17
Helical: 18-38
Extracellular: 39-193

Liu, W., Maben, Z., Wang, C., Lindquist, K. C., Li, M., Rayannavar, V., ... & Chaparro-Riggers, J. (2021). Structural delineation and phase-dependent activation of the costimulatory CD27: CD70 complex. Journal of Biological Chemistry, 297(4).

Abolhassani, H. (2021). Specific Immune Response and Cytokine Production in CD70 Deficiency. Frontiers in Pediatrics, 9, 254.

Zhang, X., Wang, Y., Zhang, D., Li, H., Zhou, Z., & Yang, R. (2020). CD70‐silenced dendritic cells induce immune tolerance in immune thrombocytopenia patients. British Journal of Haematology, 191(3), 466-475.

Tran-Nguyen, T. K., Xue, J., Feghali-Bostwick, C., Sciurba, F. C., Kass, D. J., & Duncan, S. R. (2020). CD70 activation decreases pulmonary fibroblast production of extracellular matrix proteins. American journal of respiratory cell and molecular biology, 63(2), 255-265.

Riether, C., Pabst, T., Höpner, S., Bacher, U., Hinterbrandner, M., Banz, Y., ... & Ochsenbein, A. F. (2020). Targeting CD70 with cusatuzumab eliminates acute myeloid leukemia stem cells in patients treated with hypomethylating agents. Nature medicine, 26(9), 1459-1467.

Oba, T., Hoki, T., Yamauchi, T., Keler, T., Marsh, H. C., Cao, X., & Ito, F. (2020). A critical role of CD40 and CD70 signaling in conventional type 1 dendritic cells in expansion and antitumor efficacy of adoptively transferred tumor-specific T cells. The Journal of Immunology, 205(7), 1867-1877.

Hornero, R. A., Georgiadis, C., Hua, P., Trzupek, D., He, L. Z., Qasim, W., ... & Hester, J. (2020). CD70 expression determines the therapeutic efficacy of expanded human regulatory T cells. Communications biology, 3(1), 1-17.

Bayat, P., Taghdisi, S. M., Rafatpanah, H., Abnous, K., & Ramezani, M. (2019). In vitro selection of CD70 binding aptamer and its application in a biosensor design for sensitive detection of SKOV-3 ovarian cells. Talanta, 194, 399-405.

Dhaeze, T., Tremblay, L., Lachance, C., Peelen, E., Zandee, S., Grasmuck, C., ... & Prat, A. (2019). CD70 defines a subset of proinflammatory and CNS-pathogenic TH 1/TH 17 lymphocytes and is overexpressed in multiple sclerosis. Cellular & molecular immunology, 16(7), 652-665.

O’Neill, R. E., Du, W., Mohammadpour, H., Alqassim, E., Qiu, J., Chen, G., ... & Cao, X. (2017). T Cell–Derived CD70 Delivers an Immune Checkpoint Function in Inflammatory T Cell Responses. The Journal of Immunology, 199(10), 3700-3710.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

CD70 Matched Antibody Pair (234) (CAT#: APMAB-234LY)

Cynomolgus Recombinant CD70 protein, ECD, His Tag (CAT#: V2LY-0526-LY1449)

Human Recombinant CD70 protein, ECD, AF647 Conjugated, His Tag (CAT#: V2LY-0526-LY2929)

Human Recombinant CD70 protein, Trimer, ECD, APC Conjugated, His Tag (CAT#: V2LY-0526-LY2930)

Human Recombinant CD70 protein, ECD, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2931)

Human Recombinant CD70 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2932)

Human Recombinant CD70 protein, hFc Tag (CAT#: V2LY-0526-LY2933)

Human Recombinant CD70 protein, hFc Tag-1 (CAT#: V2LY-0526-LY2934)

Human Recombinant CD70 protein, hFc Tag-2 (CAT#: V2LY-0526-LY2935)

Human Recombinant CD70 protein, Fc Tag (CAT#: V2LY-0526-LY2936)

Antibody Pairs

CD70 Matched Antibody Pair (234) (CAT#: APMAB-234LY)

Related Products

Human Recombinant CD70 protein, hFc Tag (CAT#: V2LY-0526-LY2933)

Human Anti-CD70 Recombinant Antibody (CBXC-0792) (CAT#: CBMAB-C1906-CQ)

Rat Recombinant CD70 protein, hFc Tag (CAT#: V2LY-0526-LY9266)

Human Recombinant CD70 protein, ECD, AF647 Conjugated, His Tag (CAT#: V2LY-0526-LY2929)

Human Recombinant CD70 protein, Trimer , ECD, PE Conjugated, His Tag (CAT#: V2LY-0526-LY2939)

Mouse Recombinant CD70 protein, Trimer , His Tag (CAT#: V2LY-0526-LY8064)

Mouse Recombinant CD70 protein, Fc Tag (CAT#: V2LY-0526-LY8065)

Human Recombinant CD70 protein, hFc Tag-2 (CAT#: V2LY-0526-LY2935)

Rat Anti-CD70 Recombinant Antibody (CBFYC-1412) (CAT#: CBMAB-C1470-FY)

Human Recombinant CD70 protein, rFc Tag (CAT#: V2LY-0526-LY2937)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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