Human CEACAM3 ELISA Kit (V2LY-0626-LY5556)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.048 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
27.1 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Carcinoembryonic Antigen Related Cell Adhesion Molecule 3
Function
Major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neissiria, Moxarella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. Responsible for RAC1 stimulation in the course of pathogen phagocytosis.
Biological Process
Leukocyte migration Source: Reactome
Cellular Location
Membrane
Topology
Extracellular: 35-155
Helical: 156-176
Cytoplasmic: 177-252
PTM
Tyrosine-phosphorylated in response to microbial binding. Tyr-230 and Tyr-241 are both required for phosphorylation to be detected.

Klaile, E., Salcedo, J. P. P., Klassert, T. E., Besemer, M., Bothe, A. K., Durotin, A., ... & Slevogt, H. (2021). Antibody ligation of carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), CEACAM3, and CEACAM6, differentially enhance the cytokine release of human neutrophils in responses to Candida albicans. bioRxiv.

Bonsignore, P., Kuiper, J. W., Adrian, J., Goob, G., & Hauck, C. R. (2020). CEACAM3—A Prim (at) e Invention for Opsonin-Independent Phagocytosis of Bacteria. Frontiers in immunology, 10, 3160.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CEACAM3 protein, His Tag (CAT#: V2LY-0526-LY3080)

Related Products

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Mouse Anti-CEACAM3 Recombinant Antibody (CBT2690) (CAT#: V2LY-0625-LY1529)

Mouse Anti-CEACAM3 Recombinant Antibody (CBYY-C1840) (CAT#: CBMAB-C3278-YY)

Human Recombinant CEACAM3 protein, His Tag (CAT#: V2LY-0526-LY3080)

Mouse Anti-CEACAM3 Recombinant Antibody (CBYY-C0213) (CAT#: CBMAB-C0615-YY)

Mouse Anti-CEACAM3 Recombinant Antibody (CBFYC-0069) (CAT#: CBMAB-C0078-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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