Human DCT ELISA Kit (V2LY-0626-LY1224)

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Tested Data
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Basic Information

Sensitivity
0.12 ng/mL
Detection Range
0.2-70 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
59.1 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Dopachrome Tautomerase
Function
Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA).
Biological Process
Cell development Source: Ensembl
Developmental pigmentation Source: GO_Central
Epidermis development Source: ProtInc
Melanin biosynthetic process Source: Reactome
Melanin biosynthetic process from tyrosine Source: UniProtKB
Positive regulation of neuroblast proliferation Source: GO_Central
Response to blue light Source: UniProtKB
Ventricular zone neuroblast division Source: GO_Central
Cellular Location
Melanosome membrane; Melanosome. Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38.
Topology
Lumenal, melanosome: 24-472
Helical: 473-493
Cytoplasmic: 494-519
PTM
Glycosylated.

Volk, A. E., Hedergott, A., Preising, M., Rading, S., Fricke, J., Herkenrath, P., ... & Kubisch, C. (2021). Biallelic mutations in L-dopachrome tautomerase (DCT) cause infantile nystagmus and oculocutaneous albinism. Human Genetics, 140(8), 1157-1168.

Pennamen, P., Tingaud-Sequeira, A., Gazova, I., Keighren, M., McKie, L., Marlin, S., ... & Arveiler, B. (2021). Dopachrome tautomerase variants in patients with oculocutaneous albinism. Genetics in Medicine, 23(3), 479-487.

Rosani, U., Domeneghetti, S., Maso, L., Wegner, K. M., & Venier, P. (2019). An evolutionary perspective of dopachrome tautomerase enzymes in metazoans. Genes, 10(7), 495.

Salinas‑Santander, M., Trevino, V., de la Rosa‑Moreno, E., Verduzco‑Garza, B., Sánchez‑Domínguez, C. N., Cantú‑Salinas, C., ... & Ortiz‑López, R. (2018). CAPN3, DCT, MLANA and TYRP1 are overexpressed in skin of vitiligo vulgaris Mexican patients. Experimental and Therapeutic Medicine, 15(3), 2804-2811.

Dardente, H., & Lomet, D. (2018). Photoperiod and thyroid hormone regulate expression of l‐dopachrome tautomerase (Dct), a melanocyte stem‐cell marker, in tanycytes of the ovine hypothalamus. Journal of Neuroendocrinology, 30(9), e12640.

Sultana, H., Seo, D., Choi, N. R., Bhuiyan, M. S. A., Lee, S. H., Heo, K. N., & Lee, J. H. (2018). Identification of polymorphisms in MITF and DCT genes and their associations with plumage colors in Asian duck breeds. Asian-Australasian journal of animal sciences, 31(2), 180.

Aksoy, P., & Meneses, P. I. (2017). The role of DCT in HPV16 infection of HaCaTs. PLoS One, 12(1), e0170158.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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