Human EAF2 ELISA Kit (V2LY-0626-LY3618)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.00028 ng/mL
Detection Range
0.0005-0.2 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
28.8 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
ELL associated factor 2
Research Area
Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity).

Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.
Biological Process
Apoptotic process Source: UniProtKB-KW
Negative regulation of cell growth Source: Ensembl
Negative regulation of epithelial cell proliferation involved in prostate gland development Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription elongation from RNA polymerase II promoter Source: ARUK-UCL
Cellular Location
Nucleus speckle

Pascal, L. E., Rigatti, L. H., Ai, J., Zhang, A., Zhou, J., Nelson, J. B., & Wang, Z. (2020). EAF2 loss induces prostatic intraepithelial neoplasia from luminal epithelial cells in mice. American journal of clinical and experimental urology, 8(1), 18.

Wn, K., Guo, F., Yc, F., Lv, J., Jh, F., Wang, Y., ... & Xm, M. (2020). Expression and gene regulation network of EAF2 in cervical cancer based on data mining.

Liu, R., Chen, C., Li, Y., Huang, Q., & Xue, Y. (2020). ELL-associated factors EAF1/2 negatively regulate HIV-1 transcription through inhibition of Super Elongation Complex formation. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1863(5), 194508.

Jo, Y. S., Kim, S. S., Kim, M. S., Yoo, N. J., & Lee, S. H. (2019). Candidate tumor suppressor gene EAF2 is mutated in colorectal and gastric cancers. Pathology & Oncology Research, 25(2), 823-824.

Feng, K., & Guo, H. K. (2018). Eaf2 protects human lens epithelial cells against oxidative stress‑induced apoptosis by Wnt signaling. Molecular medicine reports, 17(2), 2795-2802.

Yang, T., Jing, Y., Dong, J., Yu, X., Zhong, M., Pascal, L. E., ... & Wang, Z. (2018). Regulation of ELL2 stability and polyubiquitination by EAF2 in prostate cancer cells. The Prostate, 78(15), 1201-1212.

Zhong, M., Pascal, L. E., Cheng, E., Masoodi, K. Z., Chen, W., Green, A., ... & Wang, Z. (2018). Concurrent EAF2 and ELL2 loss phenocopies individual EAF2 or ELL2 loss in prostate cancer cells and murine prostate. American journal of clinical and experimental urology, 6(6), 234.

Zhong, M., Pascal, L., Song, Q., Chen, W., & Wang, Z. (2018, November). ELL2 and EAF2 co-regulation of AKT in prostate cancer cells. In Endocrine Abstracts (Vol. 59). Bioscientifica.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Rabbit Anti-EAF2 Recombinant Antibody (E1R8C) (CAT#: CBMAB-E0695-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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