Human GLRX3 ELISA Kit (V2LY-0626-LY3388)

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Tested Data
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Basic Information

Sensitivity
0.0089 ng/mL
Detection Range
0.02-7 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
37.4 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Glutaredoxin 3
Function
Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins (PubMed:26613676, PubMed:27519415).

Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (By similarity).

Required for hemoglobin maturation (PubMed:23615448).

Does not possess any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity.
Biological Process
[2Fe-2S] cluster assembly Source: UniProtKB
Cellular iron ion homeostasis Source: GO_Central
Negative regulation of cardiac muscle hypertrophy Source: UniProtKB
Protein maturation by iron-sulfur cluster transfer Source: UniProtKB
Regulation of the force of heart contraction Source: UniProtKB
Cellular Location
Cytosol; Cell cortex; Z line. Under the plasma membrane (By similarity). After PMA stimulation, GLRX3 and PRKCQ/PKC-theta translocate to a more extended submembrane area (By similarity). In the Z line, found associated with CSRP3 (By similarity).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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