Human HK3 ELISA Kit (V2LY-0626-LY1117)

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Tested Data
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Basic Information

Sensitivity
0.021 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
99.0 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Hexokinase 3
Function
Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed:8717435).

Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:8717435).
Biological Process
Canonical glycolysis Source: Reactome
Cellular glucose homeostasis Source: GO_Central
Fructose 6-phosphate metabolic process Source: UniProtKB
Glucose 6-phosphate metabolic process Source: UniProtKB
Glycolytic process Source: GO_Central
Cellular Location
Cytosol; Extracellular region; Mitochondrion; Ficolin-1-rich granule lumen; Secretory granule lumen

Li, T., Gu, Y., Xu, B., Kuca, K., Zhang, J., & Wu, W. (2023). CircZBTB44 promotes renal carcinoma progression by stabilizing HK3 mRNA structure. Molecular Cancer, 22(1), 1-14.

Seiler, K., Humbert, M., Minder, P., Mashimo, I., Schläfli, A. M., Krauer, D., ... & Torbett, B. E. (2022). Hexokinase 3 enhances myeloid cell survival via non-glycolytic functions. Cell death & disease, 13(5), 448.

van der Kooij, M. A., Rojas-Charry, L., Givehchi, M., Wolf, C., Bueno, D., Arndt, S., ... & Methner, A. (2022). Chronic social stress disrupts the intracellular redistribution of brain hexokinase 3 induced by shifts in peripheral glucose levels. Journal of Molecular Medicine, 100(10), 1441-1453.

Cai, T., Du, P., Suo, L., Jiang, X., Qin, Q., Song, R., ... & Zhang, J. A. (2022). High iodine promotes autoimmune thyroid disease by activating hexokinase 3 and inducing polarization of macrophages towards M1. Frontiers in Immunology, 13, 1009932.

Seiler, K., Tschan, M. P., Kalbermatter, C., Muralt, T., Rao, T. N., & Torbett, B. E. (2022). Non-Glycolytic Role of the Myeloid Hexokinase 3 (HK3) in Therapy Responses of Myeloid Malignancies. Blood, 140(Supplement 1), 5883-5883.

Xu, W., Liu, W. R., Xu, Y., Tian, X., Anwaier, A., Su, J. Q., ... & Ye, D. W. (2021). Hexokinase 3 dysfunction promotes tumorigenesis and immune escape by upregulating monocyte/macrophage infiltration into the clear cell renal cell carcinoma microenvironment. International journal of biological sciences, 17(9), 2205.

Tuo, Z., Zheng, X., Zong, Y., Li, J., Zou, C., Lv, Y., & Liu, J. (2020). HK3 is correlated with immune infiltrates and predicts response to immunotherapy in non‐small cell lung cancer. Clinical and translational medicine, 10(1), 319-330.

Lin, Y. H., Wu, Y., Wang, Y., Yao, Z. F., Tang, J., Wang, R., ... & He-Zuo, L. Ř. (2018). Spatio-temporal expression of Hexokinase-3 in the injured female rat spinal cords. Neurochemistry international, 113, 23-33.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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