Human IL3RA ELISA Kit (V2LY-0626-LY4767)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.048 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
43.3 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Interleukin 3 Receptor Subunit Alpha
Function
This is a receptor for interleukin-3.
Biological Process
Cytokine-mediated signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Membrane
Topology
Extracellular: 19-305
Helical: 306-325
Cytoplasmic: 326-378

Aisagbonhi, O., Bui, T., St. Louis, H., Pizzo, D., Meads, M., Mulholland, M., ... & Horii, M. (2023). RNA sequencing identifies human placental IL3RA as a potential predictor of adverse cardiovascular outcomes in patients with severe preeclampsia. medRxiv, 2023-06.

Qazi, S., & Uckun, F. M. (2022). Augmented expression of the IL3RA/CD123 gene in MLL/KMT2A-rearranged pediatric AML and infant ALL. Onco, 2(3), 245-263.

Yan, J., Hou, Z., Ren, Y., & Hu, J. (2022). EP300-ZNF384 Rearrangement Drive B-Cell Acute Lymphoblastic Leukemia Development By Activating IL3-IL3RA Signaling. Blood, 140(Supplement 1), 11508-11508.

Kirchhoff, D., Stelte-Ludwig, B., Lerchen, H. G., Wengner, A. M., Ahsen, O. V., Buchmann, P., ... & Sommer, A. (2020). IL3ra-targeting antibody–drug conjugate BAY-943 with a kinesin spindle protein inhibitor payload shows efficacy in preclinical models of hematologic malignancies. Cancers, 12(11), 3464.

Underwood, J. G., Smith, J. L., Call, L. F., Tseng, E., Hylkema, T. A., Ries, R. E., ... & Meshinchi, S. (2019). Discovery of Novel IL3RA (CD123) Isoforms By Long Read Transcriptomics, Heterogeneous Expression Among AML Patient Cohorts and the Implications for Anti-CD123 Therapeutics. Blood, 134, 4658.

Sommer, A., Kirchhoff, D., Wengner, A. M., Stelte-Ludwig, B., Lerchen, H. G., Rebstock, A. S., ... & Kreft, B. (2019). Anti-tumor activity of BAY-943, an anti-IL3RA ADC with a novel KSP inhibitor payload, in CDX and PDX AML models. Cancer Research, 79(13_Supplement), 4828-4828.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Canine Recombinant CD123 protein, His Tag (CAT#: V2LY-0526-LY1286)

Cynomolgus Recombinant CD123 protein, His Tag (CAT#: V2LY-0526-LY1573)

Human Recombinant IL3RA protein, ECD, His Tag (CAT#: V2LY-0526-LY4914)

Human Recombinant IL3RA protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY4915)

Human Recombinant IL3RA protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY4916)

Human Recombinant IL3RA protein, hFc Tag (CAT#: V2LY-0526-LY4917)

Human Recombinant IL3RA protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY4918)

Mouse Recombinant IL3RA protein, ECD, hFc Tag (CAT#: V2LY-0526-LY8543)

Mouse Recombinant IL3RA protein, ECD, His Tag (CAT#: V2LY-0526-LY8544)

Related Products

Human Recombinant IL3RA protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY4918)

Rat Anti-IL3RA Recombinant Antibody (9G52) (CAT#: CBMAB-C11733-LY)

Mouse Anti-IL3RA Recombinant Antibody (CBYY-C2574) (CAT#: CBMAB-C4015-YY)

Mouse Anti-IL3RA Recombinant Antibody (CBYY-I0215) (CAT#: CBMAB-I0567-YY)

Rat Anti-IL3RA Recombinant Antibody (CBYY-I1132) (CAT#: CBMAB-I2300-YY)

Rat Anti-IL3RA Recombinant Antibody (5B11) (CAT#: CBMAB-C7293-LY)

Rat Anti-IL3RA (AA 17-331) Recombinant Antibody (CBFYC-2771) (CAT#: CBMAB-C2847-FY)

Human Recombinant IL3RA protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY4915)

Rat Anti-IL3RA Recombinant Antibody (CBYY-I1409) (CAT#: CBMAB-I2579-YY)

Mouse Anti-IL3RA Recombinant Antibody (CB269) (CAT#: CBMAB-CS269LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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