Human ING1 ELISA Kit (V2LY-0626-LY4717)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0053 ng/mL
Detection Range
0.01-2 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
46.7 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Inhibitor Of Growth Family Member 1
Function
Cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. Implicated as a tumor suppressor gene.
Biological Process
Cell cycleIEA:UniProtKB-KW
Negative regulation of cell growth1 PublicationNAS:UniProtKB
Negative regulation of cell population proliferationManual Assertion Based On ExperimentTAS:ProtInc
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIBA:GO_Central
Protein import into nucleusIEA:Ensembl
Regulation of cell deathIEA:Ensembl
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
Involvement in disease
Squamous cell carcinoma of the head and neck (HNSCC):
A non-melanoma skin cancer affecting the head and neck. The hallmark of cutaneous SCC is malignant transformation of normal epidermal keratinocytes.

Yang, Y., Ma, B., Djamshidi, M., Zhang, Q., Sarkar, A., Chanda, A., ... & Riabowol, K. (2023). ING1 inhibits Twist1 expression to block EMT and is antagonized by the HDAC inhibitor vorinostat. European Journal of Cell Biology, 102(3), 151341.

Bartsch, S., Mirzakhani, K., Neubert, L., Stenzel, A., Ehsani, M., Esmaeili, M., ... & Baniahmad, A. (2021). Antithetic hTERT regulation by androgens in prostate cancer cells: hTERT inhibition is mediated by the ING1 and ING2 tumor suppressors. Cancers, 13(16), 4025.

Smolle, E., Fink-Neuboeck, N., Lindenmann, J., Smolle-Juettner, F., & Pichler, M. (2019). The biological and clinical relevance of inhibitor of growth (ING) genes in non-Small cell lung cancer. Cancers, 11(8), 1118.

Leighton, L. J., Zhao, Q., Li, X., Dai, C., Marshall, P. R., Liu, S., ... & Wei, W. (2018). A functional role for the epigenetic regulator ING1 in activity-induced gene expression in primary cortical neurons. Neuroscience, 369, 248-260.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ING1 protein, His Tag (CAT#: V2LY-0526-LY4995)

ING1 Matched Antibody Pair (641) (CAT#: APMAB-641LY)

ING1 Matched Antibody Pair (642) (CAT#: APMAB-642LY)

Antibody Pairs

ING1 Matched Antibody Pair (641) (CAT#: APMAB-641LY)

ING1 Matched Antibody Pair (642) (CAT#: APMAB-642LY)

Related Products

Rabbit Anti-ING1 Recombinant Antibody (CBYY-I1663) (CAT#: CBMAB-I2833-YY)

Mouse Anti-ING1 Recombinant Antibody (2E1) (CAT#: CBMAB-A4472-LY)

Recombinant Mouse Anti-ING1 Recombinant Antibody (Cab 1) (CAT#: CBMAB-XB0616-YC)

Mouse Anti-ING1 Recombinant Antibody (CBYY-I1053) (CAT#: CBMAB-I2221-YY)

Mouse Anti-ING1 Antibody (1C2) (CAT#: CBMAB-0530-YC)

Recombinant Mouse Anti-ING1 Recombinant Antibody (Cabs 2-9) (CAT#: CBMAB-XB0617-YC)

Mouse Anti-ING1 Recombinant Antibody (CBYY-I0246) (CAT#: CBMAB-I0645-YY)

Mouse Anti-ING1 Recombinant Antibody (1C9) (CAT#: CBMAB-I0644-YY)

ING1 Matched Antibody Pair (642) (CAT#: APMAB-642LY)

ING1 Matched Antibody Pair (641) (CAT#: APMAB-641LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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