Human KRT75 ELISA Kit (V2LY-0626-LY4779)

Name: Human KRT75 ELISA Kit
SKU: V2LY-0626-LY4779
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0053 ng/mL

Detection Range

0.01-2 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

59.6 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

keratin 75

Function

Plays a central role in hair and nail formation. Essential component of keratin intermediate filaments in the companion layer of the hair follicle.

Biological Process

Hematopoietic progenitor cell differentiationIEA:Ensembl

Cellular Location

Cytosol
Extracellular exosome
Intermediate filament
Keratin filament

Involvement in disease

Loose anagen hair syndrome (LAHS):
In LAHS, anagen hairs are easily pulled from the scalp. The hair is relatively sparse and does not grow long. Hair of fair color and hair shafts of reduced caliber, and an early age of onset are features. Usually the hairs are not fragile and there are no areas of breakage.

Deshmukh, R., Vasquez, B., Bhogadi, L., Gabe, C. M., Lukashova, L., Verdelis, K., ... & Beniash, E. (2022). Elucidating the role of keratin 75 in enamel using Krt75tm1Der knock-in mouse model. Frontiers in Physiology, 13, 2664.

Cai, C., Huang, B., Qu, K., Zhang, J., & Lei, C. (2021). A novel missense mutation within KRT75 gene strongly affects heat stress in Chinese cattle. Gene, 768, 145294.

Deshmukh, R. (2021). Role of Keratin-75 in enamel (Doctoral dissertation, University of Pittsburgh).

Mottaghitalab, F., Lanjanian, H., & Masoudi-Nejad, A. (2021). Revealing transcriptional and post-transcriptional regulatory mechanisms of γ-glutamyl transferase and keratin isoforms as novel cooperative biomarkers in low-grade glioma and glioblastoma multiforme. Genomics, 113(4), 2623-2633.

Morgan, H. J., Benketah, A., Olivero, C., Rees, E., Ziaj, S., Mukhtar, A., ... & Patel, G. K. (2020). Hair follicle differentiation‐specific keratin expression in human basal cell carcinoma. Clinical and Experimental Dermatology, 45(4), 417-425.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-KRT75 Recombinant Antibody (5F224) (CAT#: CBMAB-K0217-LY)

Mouse Anti-KRT75 Recombinant Antibody (3F9) (CAT#: CBMAB-K1992-LY)

Rat Anti-KRT75 Recombinant Antibody (8A3) (CAT#: CBMAB-K1993-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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