Human MAP4K4 ELISA Kit (V2LY-0626-LY4900)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0078 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
142.1 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4
Function
Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.
Biological Process
Intracellular signal transductionManual Assertion Based On ExperimentIDA:UniProtKB
MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:CACAO
Negative regulation of cell-matrix adhesion1 PublicationNAS:ARUK-UCL
Neuron projection morphogenesisManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of ARF protein signal transductionBy SimilarityISS:ARUK-UCL
Positive regulation of cell migrationManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of focal adhesion assemblyBy SimilarityISS:ARUK-UCL
Positive regulation of focal adhesion disassemblyBy SimilarityISS:ARUK-UCL
Positive regulation of GTPase activityBy SimilarityISS:ARUK-UCL
Positive regulation of keratinocyte migrationBy SimilarityISS:ARUK-UCL
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of JNK cascadeManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm

Patterson, V., Ullah, F., Bryant, L., Li, D., Griffin, J. N., Sidhu, A., ... & Bhoj, E. (2023). Abrogation of MAP4K4 protein function causes congenital anomalies in humans and zebrafish. Science advances, 9(17), eade0631.

González-Montero, J., Rojas, C. I., & Burotto, M. (2023). MAP4K4 and cancer: ready for the main stage?. Frontiers in Oncology, 13, 1162835.

Singh, S. K., Roy, R., Kumar, S., Srivastava, P., Jha, S., Rana, B., & Rana, A. (2023). Molecular Insights of MAP4K4 Signaling in Inflammatory and Malignant Diseases. Cancers, 15(8), 2272.

Jovanovic, D., Yan, S., & Baumgartner, M. (2022). The molecular basis of the dichotomous functionality of MAP4K4 in proliferation and cell motility control in cancer. Frontiers in oncology, 12, 1059513.

Zhang, J., Cai, X., Cui, W., & Wei, Z. (2022). Bioinformatics and Experimental Analyses Reveal MAP4K4 as a Potential Marker for Gastric Cancer. Genes, 13(10), 1786.

Singh, S. K., Kumar, S., Viswakarma, N., Principe, D. R., Das, S., Sondarva, G., ... & Rana, A. (2021). MAP4K4 promotes pancreatic tumorigenesis via phosphorylation and activation of mixed lineage kinase 3. Oncogene, 40(43), 6153-6165.

Vu, L. D., Xu, X., Zhu, T., Pan, L., van Zanten, M., de Jong, D., ... & De Smet, I. (2021). The membrane-localized protein kinase MAP4K4/TOT3 regulates thermomorphogenesis. Nature Communications, 12(1), 2842.

Fuller, S. J., Edmunds, N. S., McGuffin, L. J., Hardyman, M. A., Cull, J. J., Alharbi, H. O., ... & Clerk, A. (2021). MAP4K4 expression in cardiomyocytes: multiple isoforms, multiple phosphorylations and interactions with striatins. Biochemical Journal, 478(11), 2121-2143.

Fiedler, L. R., Chapman, K., Xie, M., Maifoshie, E., Jenkins, M., Golforoush, P. A., ... & Schneider, M. D. (2019). MAP4K4 inhibition promotes survival of human stem cell-derived cardiomyocytes and reduces infarct size in vivo. Cell Stem Cell, 24(4), 579-591.

Wu, C., Watts, M. E., & Rubin, L. L. (2019). MAP4K4 activation mediates motor neuron degeneration in amyotrophic lateral sclerosis. Cell reports, 26(5), 1143-1156.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant MAP4K4, Active protein, GST Tag (CAT#: V2LY-0526-LY5450)

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For research use only. Not intended for any clinical use.

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