Human MELTF ELISA Kit (V2LY-0626-LY3093)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.024 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
80.2 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Melanotransferrin
Function
Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.
Biological Process
Iron ion homeostasis Source: UniProtKB-KW
Iron ion transport Source: UniProtKB
Negative regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
Positive regulation of extracellular matrix disassembly Source: UniProtKB
Positive regulation of plasminogen activation Source: UniProtKB
Cellular Location
Cell membrane

Mao, Y., Wang, M., Xiong, Y., Wen, X., Zhang, M., Ma, L., & Zhang, Y. (2023). MELTF might regulate ferroptosis, pyroptosis, and autophagy in platelet-rich plasma-mediated endometrial epithelium regeneration. Reproductive Sciences, 30(5), 1506-1520.

Zhang, L., & Shi, L. (2023). The E2F1/MELTF axis fosters the progression of lung adenocarcinoma by regulating the Notch signaling pathway. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 827, 111837.

Lu, X., Wang, J., Wang, W., Lu, C., Qu, T., He, X., ... & Zhang, E. (2022). Copy number amplification and SP1-activated lncRNA MELTF-AS1 regulates tumorigenesis by driving phase separation of YBX1 to activate ANXA8 in non-small cell lung cancer. Oncogene, 41(23), 3222-3238.

Chai, J., Qin, L., Zhang, G., Hua, P., & Jin, C. (2022). Long non-coding MELTF antisense RNA 1 promotes and prognosis the progression of non-small cell lung cancer by targeting miR-1299. Bioengineered, 13(4), 10594-10604.

Ding, L., Liu, T., Qu, Y., Kang, Z., Guo, L., Zhang, H., ... & Zhang, S. (2021). lncRNA MELTF-AS1 facilitates osteosarcoma metastasis by modulating MMP14 expression. Molecular Therapy-Nucleic Acids, 26, 787-797.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant MFI2 protein, hFc Tag (CAT#: V2LY-0526-LY8663)

Mouse Recombinant MFI2 protein, His Tag (CAT#: V2LY-0526-LY8664)

Human Recombinant MFI2 protein, His Tag (CAT#: V2LY-0526-LY5530)

Related Products

Rabbit Anti-MELTF (N-terminus) Recombinant Antibody (CBFYM-2059) (CAT#: CBMAB-M2240-FY)

Rabbit Anti-MELTF Recombinant Antibody (CBFYM-2060) (CAT#: CBMAB-M2241-FY)

Mouse Anti-MELTF Recombinant Antibody (CBFYM-2057) (CAT#: CBMAB-M2238-FY)

Mouse Anti-MELTF (AA 1-302) Recombinant Antibody (CBFYM-2058) (CAT#: CBMAB-M2239-FY)

Mouse Anti-MELTF Recombinant Antibody (CBFYC-2947) (CAT#: CBMAB-C3023-FY)

Mouse Recombinant MFI2 protein, hFc Tag (CAT#: V2LY-0526-LY8663)

Mouse Anti-MELTF Recombinant Antibody (214D4) (CAT#: CBMAB-C4383-LY)

Mouse Anti-MELTF Recombinant Antibody (SHM72) (CAT#: CBMAB-C12048-LY)

Mouse Recombinant MFI2 protein, His Tag (CAT#: V2LY-0526-LY8664)

Mouse Anti-MELTF (AA 21-302) Recombinant Antibody (CBFYM-2056) (CAT#: CBMAB-M2237-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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