Mouse Recombinant MFI2 protein, His Tag (V2LY-0526-LY8664)

Name: Mouse Recombinant MFI2 protein, His Tag
SKU: V2LY-0526-LY8664
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant MFI2 protein, His Tag consist of Amino Acid: 1-708 and predicts a molecular mass of 77.5 kDa.

Molecule Mass

77.5 kDa

Verified

HPLC

Sequence

Amino Acid: 1-708

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Melanotransferrin

Function

Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.

Biological Process

Iron ion homeostasis Source: UniProtKB-KW
Iron ion transport Source: UniProtKB
Negative regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
Positive regulation of extracellular matrix disassembly Source: UniProtKB
Positive regulation of plasminogen activation Source: UniProtKB

Cellular Location

Cell membrane

Mao, Y., Wang, M., Xiong, Y., Wen, X., Zhang, M., Ma, L., & Zhang, Y. (2023). MELTF might regulate ferroptosis, pyroptosis, and autophagy in platelet-rich plasma-mediated endometrial epithelium regeneration. Reproductive Sciences, 30(5), 1506-1520.

Zhang, L., & Shi, L. (2023). The E2F1/MELTF axis fosters the progression of lung adenocarcinoma by regulating the Notch signaling pathway. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 827, 111837.

Lu, X., Wang, J., Wang, W., Lu, C., Qu, T., He, X., ... & Zhang, E. (2022). Copy number amplification and SP1-activated lncRNA MELTF-AS1 regulates tumorigenesis by driving phase separation of YBX1 to activate ANXA8 in non-small cell lung cancer. Oncogene, 41(23), 3222-3238.

Chai, J., Qin, L., Zhang, G., Hua, P., & Jin, C. (2022). Long non-coding MELTF antisense RNA 1 promotes and prognosis the progression of non-small cell lung cancer by targeting miR-1299. Bioengineered, 13(4), 10594-10604.

Ding, L., Liu, T., Qu, Y., Kang, Z., Guo, L., Zhang, H., ... & Zhang, S. (2021). lncRNA MELTF-AS1 facilitates osteosarcoma metastasis by modulating MMP14 expression. Molecular Therapy-Nucleic Acids, 26, 787-797.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant MFI2 protein, hFc Tag (CAT#: V2LY-0526-LY8663)

Human Recombinant MFI2 protein, His Tag (CAT#: V2LY-0526-LY5530)

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Mouse Recombinant MFI2 protein, hFc Tag (CAT#: V2LY-0526-LY8663)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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