Human RAPGEF4 ELISA Kit (V2LY-0626-LY5156)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.0077 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
115.5 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Rap Guanine Nucleotide Exchange Factor 4
Function
Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2 (By similarity).
Biological Process
Biological Process adaptive immune responseTAS:Reactome
Biological Process adenylate cyclase-activating G protein-coupled receptor signaling pathwayIEA:Ensembl
Biological Process calcium-ion regulated exocytosisIEA:Ensembl
Biological Process G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentTAS:ProtInc
Biological Process insulin secretionIEA:Ensembl
Biological Process positive regulation of GTPase activityManual Assertion Based On ExperimentIBA:GO_Central
Biological Process Ras protein signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of exocytosisIEA:Ensembl
Biological Process regulation of synaptic vesicle cycleIEA:Ensembl
Cellular Location
Cytoplasm
Membrane
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

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Rabbit Anti-RAPGEF4 Recombinant Antibody (D3P3J) (CAT#: CBMAB-CP2211-LY)

Mouse Anti-RAPGEF4 Recombinant Antibody (CBFYE-1616) (CAT#: CBMAB-E2275-FY)

Mouse Anti-RAPGEF4 Recombinant Antibody (1C11) (CAT#: CBMAB-R1350-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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