Human Recombinant ANGPT2 protein, His Tag-1 (V2LY-0526-LY2108)

Name: Human Recombinant ANGPT2 protein, His Tag-1
SKU: V2LY-0526-LY2108
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ANGPT2 protein, His Tag consist of Amino Acid: 275-496 and predicts a molecular mass of 27.8 kDa.

Molecule Mass

27.8 kDa

Sequence

Amino Acid: 275-496

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, CaCl2, Glycerol, Arg

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Angiopoietin 2

Function

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

Biological Process

Angiogenesis Source: GO_Central
Animal organ regeneration Source: Ensembl
Cellular response to growth factor stimulus Source: Ensembl
Gene expression Source: MGI
Germ cell development Source: Ensembl
Glomerulus vasculature development Source: UniProtKB
Leukocyte migration Source: Reactome
Maternal process involved in female pregnancy Source: Ensembl
Negative regulation of angiogenesis Source: Ensembl
Negative regulation of blood vessel endothelial cell migration Source: UniProtKB
Negative regulation of cell-substrate adhesion Source: Ensembl
Negative regulation of positive chemotaxis Source: UniProtKB
Positive regulation of angiogenesis Source: Ensembl
Response to activity Source: Ensembl
Response to glucose Source: Ensembl
Response to hypoxia Source: Ensembl
Response to mechanical stimulus Source: Ensembl
Response to organic cyclic compound Source: Ensembl
Response to radiation Source: Ensembl
Signal transduction Source: ProtInc
Tie signaling pathway Source: UniProtKB

Cellular Location

Secreted

Kamiyama, M., & Augustin, H. G. (2021). Alternatively Spliced Form of Angiopoietin-2 as a New Vascular Rheostat. Cancer Research, 81(1), 35-37.

Aarsetøy, R., Ueland, T., Aukrust, P., Michelsen, A. E., de la Fuente, R. L., Pönitz, V., ... & Nilsen, D. W. (2021). Angiopoietin‐2 and angiopoietin‐like 4 protein provide prognostic information in patients with suspected acute coronary syndrome. Journal of internal medicine.

Hultstrom, M., Fromell, K., Larsson, A., Quaggin, S. E., Betsholtz, C., Frithiof, R., ... & Jeansson, M. (2021). Elevated Angiopoietin-2 inhibits thrombomodulin-mediated anticoagulation in critically ill COVID-19 patients. medRxiv.

Xie, J. Y., Wei, J. X., Lv, L. H., Han, Q. F., Yang, W. B., Li, G. L., ... & Min, J. (2020). Angiopoietin-2 induces angiogenesis via exosomes in human hepatocellular carcinoma. Cell Communication and Signaling, 18(1), 1-13.

Pari, A. A. A., Singhal, M., Hübers, C., Mogler, C., Schieb, B., Gampp, A., ... & Felcht, M. (2020). Tumor Cell–Derived Angiopoietin-2 Promotes Metastasis in Melanoma. Cancer research, 80(12), 2586-2598.

Crist, A. M., Zhou, X., Garai, J., Lee, A. R., Thoele, J., Ullmer, C., ... & Meadows, S. M. (2019). Angiopoietin-2 inhibition rescues arteriovenous malformation in a Smad4 hereditary hemorrhagic telangiectasia mouse model. Circulation, 139(17), 2049-2063.

Schlosser, K., Taha, M., Deng, Y., McIntyre, L. A., Mei, S. H., & Stewart, D. J. (2018). High circulating angiopoietin-2 levels exacerbate pulmonary inflammation but not vascular leak or mortality in endotoxin-induced lung injury in mice. Thorax, 73(3), 248-261.

Souma, T., Thomson, B. R., Heinen, S., Carota, I. A., Yamaguchi, S., Onay, T., ... & Quaggin, S. E. (2018). Context-dependent functions of angiopoietin 2 are determined by the endothelial phosphatase VEPTP. Proceedings of the National Academy of Sciences, 115(6), 1298-1303.

Reilly, J. P., Wang, F., Jones, T. K., Palakshappa, J. A., Anderson, B. J., Shashaty, M. G., ... & Meyer, N. J. (2018). Plasma angiopoietin-2 as a potential causal marker in sepsis-associated ARDS development: evidence from Mendelian randomization and mediation analysis. Intensive care medicine, 44(11), 1849-1858.

Wu, X., Giobbie-Hurder, A., Liao, X., Connelly, C., Connolly, E. M., Li, J., ... & Hodi, F. S. (2017). Angiopoietin-2 as a biomarker and target for immune checkpoint therapy. Cancer immunology research, 5(1), 17-28.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Canine Recombinant ANGPT2 protein, His Tag (CAT#: V2LY-0526-LY1201)

Cynomolgus, Rhesus Recombinant ANGPT2 protein, hFc Tag (CAT#: V2LY-0526-LY1674)

Cynomolgus, Rhesus Recombinant ANGPT2 protein, His Tag (CAT#: V2LY-0526-LY1675)

Cynomolgus, Rhesus Recombinant ANGPT2 protein, His Tag-1 (CAT#: V2LY-0526-LY1676)

Human Recombinant ANGPT2 protein, hFc Tag (CAT#: V2LY-0526-LY2105)

Human Recombinant ANGPT2 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2106)

Human Recombinant ANGPT2 protein, His Tag (CAT#: V2LY-0526-LY2107)

Human Recombinant ANGPT2 protein, His Tag-2 (CAT#: V2LY-0526-LY2109)

Human Recombinant ANGPT2 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2110)

Human Recombinant ANGPT2 protein, Fc Tag (CAT#: V2LY-0526-LY2111)

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Human Recombinant ANGPT2 protein, hFc Tag (CAT#: V2LY-0526-LY2105)

Mouse Anti-ANGPT2 Recombinant Antibody (Angy-2-1) (CAT#: CBMAB-A2676-YC)

Human Recombinant ANGPT2 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2110)

Cynomolgus, Rhesus Recombinant ANGPT2 protein, His Tag (CAT#: V2LY-0526-LY1675)

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Human Recombinant ANGPT2 protein, Fc Tag-1 (CAT#: V2LY-0526-LY2112)

Human Recombinant ANGPT2 protein, His Tag (CAT#: V2LY-0526-LY2107)

Isotype control

For research use only. Not intended for any clinical use.

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