Human Recombinant CD86 protein, Biotin Conjugated, His & AVI Tag (V2LY-0526-LY2245)

Name: Human Recombinant CD86 protein, Biotin Conjugated, His & AVI Tag
SKU: V2LY-0526-LY2245
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His & AVI Tag

Protein Construction

This product is Human Recombinant CD86 protein, Biotin Conjugated, His & AVI Tag consist of Amino Acid: 1-239 and predicts a molecular mass of 28.42 kDa.

Molecule Mass

28.42 kDa

Conjugates

Biotin

Sequence

Amino Acid: 1-239

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD86 Molecule

Alternative Names

CD86 Molecule; CD86 Antigen (CD28 Antigen Ligand 2, B7-2 Antigen); CTLA-4 Counter-Receptor B7.2; CD28LG2; FUN-1; BU63; B70; B-Lymphocyte Activation Antigen B7-2;

Function

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824).
Also involved in the regulation of B cells function, plays a role in regulating the level of IgG1 produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).
Isoform 2: Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
(Microbial infection) Acts as a receptor for adenovirus subgroup B.

Biological Process

Activation of phospholipase C activity Source: UniProtKB
Activation of protein kinase C activity Source: UniProtKB
Adaptive immune response Source: UniProtKB-KW
B cell activation Source: UniProtKB
CD40 signaling pathway Source: UniProtKB
Cell surface receptor signaling pathway Source: GO_Central
Cellular response to lipopolysaccharide Source: GO_Central
Cytokine-mediated signaling pathway Source: Reactome
Immune response Source: GO_Central
Negative regulation of T cell proliferation Source: GO_Central
Positive regulation of cell population proliferation Source: ProtInc
Positive regulation of immunoglobulin production Source: UniProtKB
Positive regulation of interleukin-2 production Source: UniProtKB
Positive regulation of interleukin-4 production Source: UniProtKB
Positive regulation of lymphotoxin A production Source: UniProtKB
Positive regulation of NIK/NF-kappaB signaling Source: UniProtKB
Positive regulation of protein kinase B signaling Source: Reactome
Positive regulation of T cell proliferation Source: GO_Central
Positive regulation of T-helper 2 cell differentiation Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
T cell costimulation Source: GO_Central

Cellular Location

Cell membrane

Topology

Extracellular: 24-247
Helical: 248-268
Cytoplasmic: 269-329

PTM

Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

Watanabe, A., Inoue, N., Watanabe, M., Yamamoto, M., Ozaki, H., Hidaka, Y., & Iwatani, Y. (2020). Increases of CD80 and CD86 expression on peripheral blood cells and their gene polymorphisms in autoimmune thyroid disease. Immunological investigations, 49(1-2), 191-203.

Tanaka, S., Ise, W., Inoue, T., Ito, A., Ono, C., Shima, Y., ... & Kurosaki, T. (2020). Tet2 and Tet3 in B cells are required to repress CD86 and prevent autoimmunity. Nature immunology, 21(8), 950-961.

de Vos, L., Grünwald, I., Bawden, E. G., Dietrich, J., Scheckenbach, K., Wiek, C., ... & Dietrich, D. (2020). The landscape of CD28, CD80, CD86, CTLA4, and ICOS DNA methylation in head and neck squamous cell carcinomas. Epigenetics, 15(11), 1195-1212.

Haabeth, O. A. W., Blake, T. R., McKinlay, C. J., Tveita, A. A., Sallets, A., Waymouth, R. M., ... & Levy, R. (2019). Local delivery of Ox40l, Cd80, and Cd86 mRNA kindles global anticancer immunity. Cancer research, 79(7), 1624-1634.

Nombela, I., Requena-Platek, R., Morales-Lange, B., Chico, V., Puente-Marin, S., Ciordia, S., ... & Ortega-Villaizan, M. D. M. (2019). Rainbow trout red blood cells exposed to viral hemorrhagic septicemia virus up-regulate antigen-processing mechanisms and MHC I&II, CD86, and CD83 antigen-presenting cell markers. Cells, 8(5), 386.

Kleinpeter, P., Remy-Ziller, C., Winter, E., Gantzer, M., Nourtier, V., Kempf, J., ... & Marchand, J. B. (2019). By binding CD80 and CD86, the vaccinia virus M2 protein blocks their interactions with both CD28 and CTLA4 and potentiates CD80 binding to PD-L1. Journal of virology, 93(11), e00207-19.

Xiong, A., Wang, J., Mao, X. L., Jiang, Y., & Fan, Y. (2019). MiR‐199a‐3p modulates the function of dendritic cells involved in transplantation tolerance by targeting CD86. Hla, 94(6), 493-503.

Pinto, B. F., Medeiros, N. I., Teixeira-Carvalho, A., Eloi-Santos, S. M., Fontes-Cal, T., Rocha, D. A., ... & Gomes, J. A. (2018). CD86 expression by monocytes influences an immunomodulatory profile in asymptomatic patients with chronic Chagas disease. Frontiers in immunology, 9, 454.

Han, L., Shen, L., Zhu, Y., & Qiu, Y. (2017). A monoclonal antibody against CD86 and its protection in a murine lupus nephritis model of chronic graft-versus-host disease. Immunopharmacology and immunotoxicology, 39(5), 285-291.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

CD86 Matched Antibody Pair (237) (CAT#: APMAB-237LY)

CD86 Matched Antibody Pair (238) (CAT#: APMAB-238LY)

CD86 Matched Antibody Pair (239) (CAT#: APMAB-239LY)

Cynomolgus, Rhesus Recombinant CD86 protein, hFc Tag (CAT#: V2LY-0526-LY1679)

Cynomolgus, Rhesus Recombinant CD86 protein, His Tag (CAT#: V2LY-0526-LY1680)

Human Recombinant CD86 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2243)

Human Recombinant CD86 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2244)

Human Recombinant CD86 protein, His Tag (CAT#: V2LY-0526-LY2246)

Human Recombinant CD86 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2247)

Mouse Recombinant CD86 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY7839)

Antibody Pairs

CD86 Matched Antibody Pair (237) (CAT#: APMAB-237LY)

CD86 Matched Antibody Pair (238) (CAT#: APMAB-238LY)

CD86 Matched Antibody Pair (239) (CAT#: APMAB-239LY)

Related Products

Cynomolgus, Rhesus Recombinant CD86 protein, His Tag (CAT#: V2LY-0526-LY1680)

Human Recombinant CD86 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2247)

Mouse Anti-CD86 Recombinant Antibody (CBT320) (CAT#: V2LY-0625-LY3603)

Cynomolgus, Rhesus Recombinant CD86 protein, hFc Tag (CAT#: V2LY-0526-LY1679)

Rabbit Anti-CD86 Recombinant Antibody (118) (CAT#: CBMAB-C2471-LY)

Mouse Anti-CD86 Recombinant Antibody (CBXC-1863) (CAT#: CBMAB-C4998-CQ)

Rabbit Anti-CD86 Recombinant Antibody (3) (CAT#: CBMAB-C1492-LY)

Rabbit Anti-CD86 Recombinant Antibody (
1G8) (CAT#: V2LY-0725-LY644)

CD86 Matched Antibody Pair (239) (CAT#: APMAB-239LY)

Human Recombinant CD86 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2244)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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