Human Recombinant CLEC10A protein, ECD, hFc Tag (V2LY-0526-LY3219)

Name: Human Recombinant CLEC10A protein, ECD, hFc Tag
SKU: V2LY-0526-LY3219
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant CLEC10A protein, ECD, hFc Tag consist of Amino Acid: 61-292 and predicts a molecular mass of 54.6 kDa.

Molecule Mass

54.6 kDa

Protein Domain

ECD

Sequence

Amino Acid: 61-292

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

C-type lectin domain family 10, member A

Function

Probable role in regulating adaptive and innate immune responses. Binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Endocytosis Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Stimulatory C-type lectin receptor signaling pathway Source: Reactome

Cellular Location

Membrane

Topology

Cytoplasmic: 1-39
Helical: 40-60
Extracellular: 61-316

Zou, M., Wu, H., Zhou, M., Xiao, F., Abudushalamu, G., Yao, Y., ... & Wu, G. (2021). High expression of CLEC10A in head and neck squamous cell carcinoma indicates favorable prognosis and high‐level immune infiltration status. Cellular Immunology, 104472.

Eggink, L. L., Roby, K. F., Cote, R., & Hoober, J. K. (2018). An innovative immunotherapeutic strategy for ovarian cancer: CLEC10A and glycomimetic peptides. Journal for immunotherapy of cancer, 6(1), 1-16.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant CLEC10A protein, hFc Tag (CAT#: V2LY-0526-LY8123)

Mouse Recombinant CLEC10A protein, His Tag (CAT#: V2LY-0526-LY8124)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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