Human Recombinant CXCL14 protein (V2LY-0526-LY2405)

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Basic Information

Expressed Host
E. coli
Protein Species
Human
Protein Construction
This product is Human Recombinant CXCL14 protein consist of Amino Acid: 35-111 and predicts a molecular mass of 9.4 kDa.
Molecule Mass
9.4 kDa
Sequence
Amino Acid: 35-111
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>90% as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
C-X-C Motif Chemokine Ligand 14
Function
Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.
Biological Process
Antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
Cell-cell signaling Source: ProtInc
Chemotaxis Source: ProtInc
Inner ear development Source: Ensembl
Killing of cells of other organism Source: UniProtKB
Negative regulation of myoblast differentiation Source: Ensembl
Positive regulation of natural killer cell chemotaxis Source: Ensembl
Signal transduction Source: ProtInc
Cellular Location
Secreted
PTM
Ubiquitinated, followed by degradation by the proteasome.

Chen, L., Lei, Y., & Zhang, L. (2021). Role of CXC motif chemokine ligand 14 promoter region DNA methylation and single nucleotide polymorphism in influenza A severity. Respiratory Medicine, 185, 106462.

Matsushita, Y., Hasegawa, Y., Takebe, N., Onodera, K., Shozushima, M., Oda, T., ... & Ishigaki, Y. (2021). Serum C‐X‐C motif chemokine ligand 14 levels are associated with serum C‐peptide and fatty liver index in type 2 diabetes mellitus patients. Journal of Diabetes Investigation, 12(6), 1042-1049.

Wang, C., Zhao, N., Sato, F., Tanimoto, K., Okada, H., Liu, Y., & Bhawal, U. K. (2021). The roles of Y-box-binding protein (YB)-1 and CXC motif chemokine ligand 14 (CXCL14) in the progression of prostate cancer via extracellular-signal-regulated kinase (ERK) signaling. Bioengineered, 12(2), 9128-9139.

Cereijo, R., Quesada-Lopez, T., Gavalda-Navarro, A., Tarascó, J., Pellitero, S., Reyes, M., ... & Villarroya, F. (2021). The chemokine CXCL14 is negatively associated with obesity and concomitant type-2 diabetes in humans. International Journal of Obesity, 45(3), 706-710.

Qi, J., Li, J., Wang, Y., Wang, W., Zhu, Q., He, Y., ... & Sun, Y. (2021). Novel role of CXCL14 in modulating STAR expression in luteinized granulosa cells: implication for progesterone synthesis in PCOS patients. Translational Research, 230, 55-67.

García-Beltran, C., Cereijo, R., Quesada-López, T., Malpique, R., López-Bermejo, A., de Zegher, F., ... & Villarroya, F. (2020). Reduced circulating levels of chemokine CXCL14 in adolescent girls with polycystic ovary syndrome: normalization after insulin sensitization. BMJ Open Diabetes Research and Care, 8(1), e001035.

Ko, H. M., Moon, J. S., Shim, H. K., Lee, S. Y., Kang, J. H., Kim, M. S., ... & Kim, S. H. (2020). Inhibitory effect of CXC motif chemokine ligand 14 on the osteogenic differentiation of human periodontal ligament cells through transforming growth factor-beta1. Archives of Oral Biology, 115, 104733.

Yang, X. Y., Ozawa, S., Kato, Y., Maehata, Y., Izukuri, K., Ikoma, T., ... & Hata, R. I. (2019). CXC motif chemokine ligand 14 is a unique multifunctional regulator of tumor progression. International Journal of Molecular Sciences, 20(8), 1872.

Li, L., Li, Q., Wei, L., Wang, Z., Ma, W., Liu, F., ... & Qian, Y. (2019). Chemokine (C‐X‐C motif) ligand 14 contributes to lipopolysaccharide‐induced fibrogenesis in mouse L929 fibroblasts via modulating PPM1A. Journal of Cellular Biochemistry, 120(8), 13372-13381.

Cereijo, R., Gavaldà-Navarro, A., Cairó, M., Quesada-López, T., Villarroya, J., Morón-Ros, S., ... & Villarroya, F. (2018). CXCL14, a brown adipokine that mediates brown-fat-to-macrophage communication in thermogenic adaptation. Cell metabolism, 28(5), 750-763.

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For research use only. Not intended for any clinical use.

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