Human Recombinant DEFB1 protein, hFc Tag (V2LY-0526-LY2334)

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Basic Information

Expressed Host
HEK293 Cells
Protein Species
Human
Tag
hFc Tag
Protein Construction
This product is Human Recombinant DEFB1 protein, hFc Tag consist of Amino Acid: 1-68 and predicts a molecular mass of 31.8 kDa.
Molecule Mass
31.8 kDa
Sequence
Amino Acid: 1-68
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by SDS-PAGE.
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Defensin Beta 1
Function
Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed:25122636).
Biological Process
Antibacterial humoral response Source: UniProtKB
Antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
Antimicrobial humoral response Source: Reactome
Calcium-mediated signaling using intracellular calcium source Source: UniProtKB
cAMP-mediated signaling Source: UniProtKB
Chemotaxis Source: ProtInc
Defense response to bacterium Source: MGI
Defense response to Gram-negative bacterium Source: UniProtKB
Defense response to Gram-positive bacterium Source: UniProtKB
G protein-coupled receptor signaling pathway Source: ProtInc
Immune response Source: ProtInc
Innate immune response Source: UniProtKB
Innate immune response in mucosa Source: UniProtKB
Positive regulation of flagellated sperm motility involved in capacitation Source: UniProtKB
Response to bacterium Source: UniProtKB
Cellular Location
Secreted; Membrane. Associates with tumor cell membrane-derived microvesicles (PubMed:23938203).

Li, S., Li, H., Xu, Y., Ning, W., Hu, S., Wei, S., ... & Liu, M. (2022). Implications of Human Antimicrobial Peptide Defensin Beta-1 in Clinical Oral Squamous Cell Carcinoma Patients via an Integrated Bioinformatics Approach. Computational and Mathematical Methods in Medicine, 2022.

Ślebioda, Z., Woźniak, T., Dorocka‐Bobkowska, B., Woźniewicz, M., & Kowalska, A. (2021). Beta‐defensin 1 gene polymorphisms in the pathologies of the oral cavity—Data from meta‐analysis: Association only with rs1047031 not with rs1800972, rs1799946, and rs11362. Journal of Oral Pathology & Medicine, 50(1), 22-31.

Singh, J. K. J., Vaithilingam, R. D., Ng, C. C., Baharuddin, N. A., Safii, S. H., & Rahman, M. T. (2021). Prostaglandin-endoperoxide synthase (PTGS2) and Defensin beta 1 (DEFB1) gene polymorphisms are not associated with periodontitis in Malays. The Malaysian journal of pathology, 43(3), 425-434.

Salem, R. M., Abdelrahman, A. M. N., Abd El‐Kareem, H. M., & Seif, M. (2021). DEFB1 gene polymorphisms modify vitiligo extent and response to NB‐UVB phototherapy. Dermatologic Therapy, 34(3), e14921.

Antunes, L. S., Carvalho, L., Petean, I. B. F., Antunes, L. A., Freitas, J. V., Salles, A. G., ... & Sousa‐Neto, M. D. (2021). Association between genetic polymorphisms in the promoter region of the defensin beta 1 gene and persistent apical periodontitis. International Endodontic Journal, 54(1), 38-45.

Hatipoğlu, Ö., & Saydam, F. (2020). Association between rs11362 polymorphism in the beta-defensin 1 (DEFB1) gene and dental caries: A meta-analysis. Journal of Oral Biosciences, 62(3), 272-279.

Subbiah, H. V., Babu, P. R., & Subbiah, U. (2020). In silico analysis of non-synonymous single nucleotide polymorphisms of human DEFB1 gene. Egyptian Journal of Medical Human Genetics, 21(1), 1-9.

Kallel, A., Salem, T. B., Hammami, M. B., Said, F., Jemaa, R., Houman, M. H., & Feki, M. (2019). Association of systemic beta-defensin-1 and-20G/A DEFB1 gene polymorphism with Behcet's disease. European journal of internal medicine, 65, 58-62.

Liu, D., Ray, B., Neavin, D. R., Zhang, J., Athreya, A. P., Biernacka, J. M., ... & Weinshilboum, R. M. (2018). Beta-defensin 1, aryl hydrocarbon receptor and plasma kynurenine in major depressive disorder: metabolomics-informed genomics. Translational psychiatry, 8(1), 1-13.

Lips, A., Antunes, L. S., Antunes, L. A., de Abreu, J. G. B., Barreiros, D., de Oliveira, D. S. B., ... & Küchler, E. C. (2017). Genetic polymorphisms in DEFB1 and miRNA202 are involved in salivary human β-defensin 1 levels and caries experience in children. Caries Research, 51(3), 209-215.

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For research use only. Not intended for any clinical use.

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