Human Recombinant EB3 protein, His Tag (V2LY-0526-LY3669)

Name: Human Recombinant EB3 protein, His Tag
SKU: V2LY-0526-LY3669
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant EB3 protein, His Tag consist of Amino Acid: 1-281 and predicts a molecular mass of 33.8 kDa.

Molecule Mass

33.8 kDa

Sequence

Amino Acid: 1-281

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Microtubule Associated Protein RP/EB Family Member 3

Function

Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570).
Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570).
May play a role in cell migration (By similarity).

Biological Process

Cell divisionIEA:UniProtKB-KW
Positive regulation of cyclin-dependent protein serine/threonine kinase activityBy SimilarityISS:BHF-UCL
Positive regulation of microtubule plus-end bindingIEA:Ensembl
Positive regulation of protein kinase activityManual Assertion Based On ExperimentIDA:ARUK-UCL
Positive regulation of transcription, DNA-templatedBy SimilarityISS:BHF-UCL
Protein localizationManual Assertion Based On ExperimentTAS:ARUK-UCL
Protein localization to microtubule plus-endManual Assertion Based On ExperimentIBA:GO_Central
Regulation of microtubule polymerizationManual Assertion Based On ExperimentIMP:ARUK-UCL
Regulation of microtubule polymerization or depolymerizationManual Assertion Based On ExperimentIBA:GO_Central
Spindle assemblyManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm, cytoskeleton
Associated with the microtubule network. Detected at the plus end of microtubules.

Zhang, Y., Liu, F., Zheng, J., Jiang, K., Ai, H., Liu, L., & Mao, D. (2024). MAPRE3 as an epigenetic target of EZH2 restricts ovarian cancer proliferation in vitro and in vivo. Experimental Cell Research, 113913.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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