Human Recombinant FGF4 protein (V2LY-0526-LY4060)

Name: Human Recombinant FGF4 protein
SKU: V2LY-0526-LY4060
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Protein Construction

This product is Human Recombinant FGF4 protein consist of Amino Acid: 54-206 and predicts a molecular mass of 16.92 kDa.

Molecule Mass

16.92 kDa

Verified

HPLC

Sequence

Amino Acid: 54-206

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

fibroblast growth factor 4

Research Area

Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal limb and cardiac valve development during embryogenesis.

Biological Process

Animal organ morphogenesis Source: GO_Central
Apoptotic process involved in morphogenesis Source: Ensembl
Cartilage condensation Source: Ensembl
Cell-cell signaling Source: ProtInc
Cell differentiation Source: GO_Central
Cellular response to leukemia inhibitory factor Source: Ensembl
Chondroblast differentiation Source: UniProtKB
Cranial suture morphogenesis Source: Ensembl
Embryonic hindlimb morphogenesis Source: Ensembl
Fibroblast growth factor receptor signaling pathway Source: MGI
Mesenchymal cell proliferation Source: UniProtKB
Negative regulation of apoptotic process Source: Ensembl
Odontogenesis of dentin-containing tooth Source: Ensembl
Positive regulation of cell division Source: UniProtKB-KW
Positive regulation of cell population proliferation Source: MGI
Positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
Positive regulation of gene expression Source: GO_Central
Positive regulation of protein phosphorylation Source: GO_Central
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Regulation of cell migration Source: GO_Central
Regulation of endothelial cell chemotaxis to fibroblast growth factor Source: UniProtKB
Signal transduction Source: ProtInc
Stem cell population maintenance Source: Ensembl

Cellular Location

Secreted

Hongwei, L., Juan, L., Xiaoying, X., & Zhijun, F. (2022). MicroRNA-106b-5p (miR-106b-5p) suppresses the proliferation and metastasis of cervical cancer cells via down-regulating fibroblast growth factor 4 (FGF4) expression. Cytotechnology, 1-10.

Yilmaz, F., Micili, S. C., & Erbil, G. (2022). The role of FGF-4 and FGFR-2 on preimplantation embryo development in experimental maternal diabetes. Gynecological Endocrinology, 38(3), 248-252.

Son, J., Tae, J. Y., Min, S. K., Ko, Y., & Park, J. B. (2020). Fibroblast growth factor-4 maintains cellular viability while enhancing osteogenic differentiation of stem cell spheroids in part by regulating RUNX2 and BGLAP expression. Experimental and Therapeutic Medicine, 20(3), 2013-2020.

Kumagai, Y., Kikuchi, T., Nonaka, A., Hiraide, M., Sato, S., Sakuraoka, M., ... & Kobayashi, M. (2019). Site-directed mutagenesis of cysteine to serine residues affects heparin binding and mitogenicity in fibroblast growth factor 4 produced in Escherichia coli. Biotechnology & Biotechnological Equipment, 33(1), 498-503.

Kim, J., Kim, H. S., Shim, J. J., Lee, J., Kim, A. Y., & Kim, J. (2019). Critical role of the fibroblast growth factor signalling pathway in Ewing's sarcoma octamer‐binding transcription factor 4‐mediated cell proliferation and tumorigenesis. The FEBS Journal, 286(22), 4443-4472.

Chen, D., & Lee, T. H. (2019). New oncogenic signalling pathway: EWS‐Oct4 mediates bone and soft tissue tumourigenesis by activating fibroblast growth factor‐4. The FEBS Journal, 286(22), 4418-4421.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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