Human Recombinant GALNT2 protein, His Tag (V2LY-0526-LY4255)

Name: Human Recombinant GALNT2 protein, His Tag
SKU: V2LY-0526-LY4255
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant GALNT2 protein, His Tag consist of Amino Acid: 52-571 and predicts a molecular mass of 61.6 kDa.

Molecule Mass

61.6 kDa

Verified

HPLC

Sequence

Amino Acid: 52-571

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Polypeptide N-Acetylgalactosaminyltransferase 2

Function

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b. Probably involved in O-linked glycosylation of the immunoglobulin A1 (IgA1) hinge region. Involved in O-linked glycosylation of APOC-III, ANGPTL3 and PLTP. It participates in the regulation of HDL-C metabolism (PubMed:27508872, PubMed:32293671).

Biological Process

O-glycan processing Source: GO_Central
Protein maturation Source: BHF-UCL
Protein O-linked glycosylation Source: UniProtKB
Protein O-linked glycosylation via serine Source: BHF-UCL
Protein O-linked glycosylation via threonine Source: BHF-UCL

Cellular Location

Secreted; Golgi stack membrane. Resides preferentially in the trans and medial parts of the Golgi stack. A secreted form also exists.

Topology

Cytoplasmic: 1-6
Helical: 7-24
Lumenal: 25-571

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human GALNT2 ELISA Kit (CAT#: V2LY-0626-LY5741)

Related Products

Mouse Anti-GALNT2 Recombinant Antibody (1501421) (CAT#: CBMAB-G1861-LY)

Mouse Anti-GALNT2 Recombinant Antibody (G1861) (CAT#: V2LY-1225-LY628)

Human GALNT2 ELISA Kit (CAT#: V2LY-0626-LY5741)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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