Human Recombinant GSTM2 protein, His Tag (V2LY-0526-LY4436)

Name: Human Recombinant GSTM2 protein, His Tag
SKU: V2LY-0526-LY4436
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant GSTM2 protein, His Tag consist of Amino Acid: 1-218 and predicts a molecular mass of 27.2 kDa.

Molecule Mass

27.2 kDa

Sequence

Amino Acid: 1-218

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>97% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

GSTM2

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276).

Biological Process

Cellular detoxification of nitrogen compound Source: BHF-UCL
Cellular response to caffeine Source: BHF-UCL
Glutathione metabolic process Source: UniProtKB
Hepoxilin biosynthetic process Source: UniProtKB
Linoleic acid metabolic process Source: BHF-UCL
Negative regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
Nitrobenzene metabolic process Source: BHF-UCL
Positive regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
Regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Source: BHF-UCL
Regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: BHF-UCL
Regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion Source: BHF-UCL
Relaxation of cardiac muscle Source: BHF-UCL
Xenobiotic catabolic process Source: UniProtKB

Cellular Location

Cytoplasm

Li, C., Liu, J., He, D., Mao, F., Rao, X., Zhao, Y., ... & Liu, X. (2022). GSTM2 is a key molecular determinant of resistance to SG-ARIs. Oncogene, 41(40), 4498-4511.

Zhang, W., Shi, Y., Niu, S., Li, L., Lin, L., Gao, X., ... & Dai, Y. (2022). Integrated computer analysis and a self-built Chinese cohort study identified GSTM2 as one survival-relevant gene in human colon cancer potentially regulating immune microenvironment. Frontiers in Oncology, 12, 881906.

Peng, L., Zhuang, L., Lin, K., Yao, Y., Zhang, Y., Arumugam, T., ... & Huang, H. (2021). Downregulation of GSTM2 enhances gemcitabine chemosensitivity of pancreatic cancer in vitro and in vivo. Pancreatology, 21(1), 115-123.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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