Human Recombinant GSTZ1 protein, His Tag (V2LY-0526-LY4439)

Name: Human Recombinant GSTZ1 protein, His Tag
SKU: V2LY-0526-LY4439
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant GSTZ1 protein, His Tag consist of Amino Acid: 1-216 and predicts a molecular mass of 26 kDa.

Molecule Mass

26 kDa

Sequence

Amino Acid: 1-216

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile HEPES, EDTA, Glycerol, Tween

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Glutathione S-Transferase Zeta 1

Function

Bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with T-butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid.

Biological Process

Glutathione metabolic process Source: UniProtKB
L-phenylalanine catabolic process Source: GO_Central
Tyrosine catabolic process Source: UniProtKB-KW

Cellular Location

Cytoplasm

Involvement in disease

Maleylacetoacetate isomerase deficiency (MAAID):
An autosomal recessive inborn error of metabolism characterized by mild elevations in succinylacetone in blood and urine, usually identified by newborn screening. Liver function and coagulation are normal. MAAID is a benign disorder.

Liao, Y., Chin Chan, S., Welsh, E. A., Fang, B., Sun, L., Schönbrunn, E., ... & Rix, U. (2023). Chemical Proteomics with Novel Fully Functionalized Fragments and Stringent Target Prioritization Identifies the Glutathione-Dependent Isomerase GSTZ1 as a Lung Cancer Target. ACS Chemical Biology.

Wang, Q., Bin, C., Xue, Q., Gao, Q., Huang, A., Wang, K., & Tang, N. (2021). GSTZ1 sensitizes hepatocellular carcinoma cells to sorafenib-induced ferroptosis via inhibition of NRF2/GPX4 axis. Cell death & disease, 12(5), 426.

Alaidaroos, N. Y., Alraies, A., Waddington, R. J., Sloan, A. J., & Moseley, R. (2021). Differential SOD2 and GSTZ1 profiles contribute to contrasting dental pulp stem cell susceptibilities to oxidative damage and premature senescence. Stem Cell Research & Therapy, 12(1), 1-19.

Lei, C., Wang, Q., Tang, N., & Wang, K. (2020). GSTZ1‐1 downregulates Wnt/β‐catenin signalling in hepatocellular carcinoma cells. FEBS Open bio, 10(1), 6-17.

Li, J., Wang, Q., Yang, Y., Lei, C., Yang, F., Liang, L., ... & Tang, N. (2019). GSTZ1 deficiency promotes hepatocellular carcinoma proliferation via activation of the KEAP1/NRF2 pathway. Journal of Experimental & Clinical Cancer Research, 38(1), 1-17.

Yang, F., Li, J., Deng, H., Wang, Y., Lei, C., Wang, Q., ... & Tang, N. (2019). GSTZ 1‐1 Deficiency Activates NRF 2/IGF 1R Axis in HCC via Accumulation of Oncometabolite Succinylacetone. The EMBO Journal, 38(15), e101964.

Yang, P., Cao, W. C., Zhou, B., Zheng, T. Z., Deng, Y. L., Luo, Q., ... & Lu, W. Q. (2019). Urinary biomarker of prenatal exposure to disinfection byproducts, maternal genetic polymorphisms in CYP2E1 and GSTZ1, and birth outcomes. Environmental Science & Technology, 53(20), 12026-12034.

Tian, D. D., Bennett, S. K., Coupland, L. A., Forwood, K., Lwin, Y., Pooryousef, N., ... & Blackburn, A. C. (2019). GSTZ1 genotypes correlate with dichloroacetate pharmacokinetics and chronic side effects in multiple myeloma patients in a pilot phase 2 clinical trial. Pharmacology research & perspectives, 7(6), e00526.

Langaee, T., Wagner, R., Horne, L. P., Lawson, L. A., Becker, C., Shahin, M., ... & Stacpoole, P. W. (2018). Personalized dosing of dichloroacetate using GSTZ1 clinical genotyping assay. Genetic testing and molecular biomarkers, 22(4), 266-269.

Zhou, B., Yang, P., Gong, Y. J., Zeng, Q., Lu, W. Q., & Miao, X. P. (2018). Effect modification of CPY2E1 and GSTZ1 genetic polymorphisms on associations between prenatal disinfection by-products exposure and birth outcomes. Environmental Pollution, 243, 1126-1133.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

GSTZ1 Matched Antibody Pair (513) (CAT#: APMAB-513LY)

GSTZ1 Matched Antibody Pair (514) (CAT#: APMAB-514LY)

Antibody Pairs

GSTZ1 Matched Antibody Pair (513) (CAT#: APMAB-513LY)

GSTZ1 Matched Antibody Pair (514) (CAT#: APMAB-514LY)

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GSTZ1 Matched Antibody Pair (513) (CAT#: APMAB-513LY)

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GSTZ1 Matched Antibody Pair (514) (CAT#: APMAB-514LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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