Human Recombinant HHLA2 protein, ECD, hFc Tag (V2LY-0526-LY2277)

Name: Human Recombinant HHLA2 protein, ECD, hFc Tag
SKU: V2LY-0526-LY2277
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant HHLA2 protein, ECD, hFc Tag consist of Amino Acid: 1-344 and predicts a molecular mass of 63.7 kDa.

Molecule Mass

63.7 kDa

Protein Domain

ECD

Sequence

Amino Acid: 1-344

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Herv-H Ltr-Associating 2

Function

Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.

Biological Process

Positive regulation of activated T cell proliferation Source: UniProtKB
Positive regulation of cytokine production Source: UniProtKB
Regulation of cytokine production Source: GO_Central
T cell costimulation Source: UniProtKB
T cell receptor signaling pathway Source: GO_Central

Cellular Location

Membrane

Topology

Helical: 345-365

Mortezaee, K. (2023). HHLA2 immune-regulatory roles in cancer. Biomedicine & Pharmacotherapy, 162, 114639.

Li, Y., Lv, C., Yu, Y., Wu, B., Zhang, Y., Lang, Q., ... & Tian, Y. (2022). KIR3DL3-HHLA2 and TMIGD2-HHLA2 pathways: The dual role of HHLA2 in immune responses and its potential therapeutic approach for cancer immunotherapy. Journal of advanced research.

Wei, Y., Ren, X., Galbo Jr, P. M., Moerdler, S., Wang, H., Sica, R. A., ... & Zang, X. (2021). KIR3DL3-HHLA2 is a human immunosuppressive pathway and a therapeutic target. Science immunology, 6(61), eabf9792.

Bhatt, R. S., Berjis, A., Konge, J. C., Mahoney, K. M., Klee, A. N., Freeman, S. S., ... & Freeman, G. J. (2021). KIR3DL3 is an inhibitory receptor for HHLA2 that mediates an alternative immunoinhibitory pathway to PD1. Cancer immunology research, 9(2), 156-169.

Luo, M., Lin, Y., Liang, R., Li, Y., & Ge, L. (2021). Clinical significance of the HHLA2 protein in hepatocellular carcinoma and the tumor microenvironment. Journal of Inflammation Research, 14, 4217.

Zhou, Q. H., Li, K. W., Chen, X., He, H. X., Peng, S. M., Peng, S. R., ... & Huang, H. (2020). HHLA2 and PD-L1 co-expression predicts poor prognosis in patients with clear cell renal cell carcinoma. Journal for immunotherapy of cancer, 8(1).

Chen, L., Zhu, D., Feng, J., Zhou, Y., Wang, Q., Feng, H., ... & Jiang, J. (2019). Overexpression of HHLA2 in human clear cell renal cell carcinoma is significantly associated with poor survival of the patients. Cancer cell international, 19(1), 1-12.

Jing, C. Y., Fu, Y. P., Yi, Y., Zhang, M. X., Zheng, S. S., Huang, J. L., ... & Zhang, B. H. (2019). HHLA2 in intrahepatic cholangiocarcinoma: an immune checkpoint with prognostic significance and wider expression compared with PD-L1. Journal for Immunotherapy of Cancer, 7, 1-11.

Shimonosono, M., Arigami, T., Yanagita, S., Matsushita, D., Uchikado, Y., Kijima, Y., ... & Natsugoe, S. (2018). The association of human endogenous retrovirus-H long terminal repeat-associating protein 2 (HHLA2) expression with gastric cancer prognosis. Oncotarget, 9(31), 22069.

Zhu, Z., & Dong, W. (2018). Overexpression of HHLA2, a member of the B7 family, is associated with worse survival in human colorectal carcinoma. OncoTargets and therapy, 1563-1570.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant HHLA2 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY1388)

Cynomolgus Recombinant HHLA2 protein, His Tag (CAT#: V2LY-0526-LY1389)

Human Recombinant HHLA2 protein, ECD, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2278)

Human Recombinant HHLA2 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2279)

Human Recombinant HHLA2 protein, His Tag (CAT#: V2LY-0526-LY2280)

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Cynomolgus Recombinant HHLA2 protein, His Tag (CAT#: V2LY-0526-LY1389)

Human Recombinant HHLA2 protein, His Tag (CAT#: V2LY-0526-LY2280)

Human Recombinant HHLA2 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2279)

Cynomolgus Recombinant HHLA2 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY1388)

Mouse Anti-HHLA2 Recombinant Antibody (CBYY-1762) (CAT#: CBMAB-2111-YY)

Human Recombinant HHLA2 protein, ECD, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2278)

Rat Anti-HHLA2 Recombinant Antibody (CBYY-1067) (CAT#: CBMAB-1071-YY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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