Human Recombinant IL32 (isoform alpha) protein, His Tag (V2LY-0526-LY4896)

Name: Human Recombinant IL32 (isoform alpha) protein, His Tag
SKU: V2LY-0526-LY4896
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant IL32 (isoform alpha) protein, His Tag consist of Amino Acid: 1-131 and predicts a molecular mass of 15.7 kDa.

Molecule Mass

15.7 kDa

Sequence

Amino Acid: 1-131

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Interleukin 32

Function

Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.

Biological Process

Cell adhesionManual Assertion Based On ExperimentTAS:ProtInc
Defense responseManual Assertion Based On ExperimentTAS:ProtInc
Immune responseManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of viral life cycleIDA:ARUK-UCL
Positive regulation of gene expressionIDA:ARUK-UCL
Positive regulation of RNA polymerase II regulatory region sequence-specific DNA bindingIDA:ARUK-UCL
Positive regulation of type III interferon productionIDA:ARUK-UCL

Cellular Location

Secreted

Frost, B., Schmidt, M., Klein, B., Loeffler‐Wirth, H., Krohn, K., Reidenbach, T., ... & Kunz, M. (2023). Single‐cell transcriptomics reveals prominent expression of IL‐14, IL‐18, and IL‐32 in psoriasis. European Journal of Immunology, 2250354.

Sun, Y., Qian, Y., Chen, C., Wang, H., Zhou, X., Zhai, W., ... & Gao, Y. (2022). Extracellular vesicle IL-32 promotes the M2 macrophage polarization and metastasis of esophageal squamous cell carcinoma via FAK/STAT3 pathway. Journal of Experimental & Clinical Cancer Research, 41(1), 1-22.

Aass, K. R., Mjelle, R., Kastnes, M. H., Tryggestad, S. S., van den Brink, L. M., Roseth, I. A., ... & Standal, T. (2022). Intracellular IL-32 regulates mitochondrial metabolism, proliferation, and differentiation of malignant plasma cells. Iscience, 25(1).

Aass, K. R., Kastnes, M. H., & Standal, T. (2021). Molecular interactions and functions of IL-32. Journal of Leucocyte Biology, 109(1), 143-159.

Gautam, A., & Pandit, B. (2021). IL32: The multifaceted and unconventional cytokine. Human Immunology, 82(9), 659-667.

Mazlum, F., Gharesi-Fard, B., Hadinedoushan, H., & Bakhshizadeh Ghashti, Y. (2021). Association between interleukin-32 gene polymorphism and susceptibility to preeclampsia: Running Head: Association of IL-32 and preeclampsia. Hypertension in Pregnancy, 40(3), 218-225.

de Lima Silva, L. L., Gomes, R. S., Silva, M. V. T., Joosten, L. A., & Ribeiro-Dias, F. (2020). IL-15 enhances the capacity of primary human macrophages to control Leishmania braziliensis infection by IL-32/vitamin D dependent and independent pathways. Parasitology international, 76, 102097.

Wen, S., Hou, Y., Fu, L., Xi, L., Yang, D., Zhao, M., ... & Liu, M. (2019). Cancer-associated fibroblast (CAF)-derived IL32 promotes breast cancer cell invasion and metastasis via integrin β3–p38 MAPK signalling. Cancer letters, 442, 320-332.

Dos Santos, J. C., de Figueiredo, A. M. B., Silva, M. V. T., Cirovic, B., de Bree, L. C. J., Damen, M. S., ... & Joosten, L. A. (2019). β-Glucan-induced trained immunity protects against Leishmania braziliensis infection: a crucial role for IL-32. Cell reports, 28(10), 2659-2672.

Sloot, Y. J., Smit, J. W., Joosten, L. A., & Netea-Maier, R. T. (2018, August). Insights into the role of IL-32 in cancer. In Seminars in immunology (Vol. 38, pp. 24-32). Academic Press.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

IL32 Matched Antibody Pair (634) (CAT#: APMAB-634LY)

Antibody Pairs

IL32 Matched Antibody Pair (634) (CAT#: APMAB-634LY)

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IL32 Matched Antibody Pair (634) (CAT#: APMAB-634LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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