Human Recombinant LTA4H protein, His Tag (V2LY-0526-LY5287)

Name: Human Recombinant LTA4H protein, His Tag
SKU: V2LY-0526-LY5287
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant LTA4H protein, His Tag consist of Amino Acid: 1-611 and predicts a molecular mass of 70.7 kDa.

Molecule Mass

70.7 kDa

Verified

HPLC

Sequence

Amino Acid: 1-611

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

leukotriene A4 hydrolase

Function

Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities. Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the pro-inflammatory mediator leukotriene B4 (LTB4) (PubMed:11917124, PubMed:12207002, PubMed:15078870, PubMed:18804029, PubMed:1897988, PubMed:1975494, PubMed:2244921).
Has also aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides (PubMed:20813919, PubMed:18804029).
In addition to its pro-inflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL) (PubMed:20813919, PubMed:24591641).
Involved also in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, two lipid mediators that show potent anti-inflammatory and pro-resolving actions (PubMed:21206090).

Biological Process

Cellular protein metabolic processManual Assertion Based On ExperimentIMP:CAFA
Leukotriene biosynthetic processManual Assertion Based On ExperimentIDA:UniProtKB
Lipid metabolic processIEA:UniProtKB-KW
Peptide catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Response to peptide hormoneIEA:Ensembl
Response to zinc ionIEA:Ensembl
Type I pneumocyte differentiationIEA:Ensembl

Cellular Location

Cytoplasm

PTM

Phosphorylation at Ser-416 inhibits leukotriene-A4 hydrolase activity.

Ren, T., Wang, S., Zhang, B., Zhou, W., Wang, C., Zhao, X., & Feng, J. (2023). LTA4H extensively associates with mRNAs and lncRNAs indicative of its novel regulatory targets. PeerJ, 11, e14875.

Wan, X., Wang, H., Shi, B., Guo, Y., Liu, S. Y., & Wang, X. (2023). An enzyme activated fluorescent probe for LTA4H activity sensing and its application in cancer screening. Talanta, 253, 123887.

Kumar, S. S., Solomon, R., Gautam, P., Inbaraj, L. R., Sivadasan, A., Michael, J. S., ... & Manesh, A. (2023). Leukotriene A4 hydrolase (LTA4H rs17525495) gene polymorphisms and paradoxical reactions in extrapulmonary tuberculosis. Scientific Reports, 13(1), 3746.

Fenanir, F., Semmeq, A., Benguerba, Y., Badawi, M., Dziurla, M. A., Amira, S., & Laouer, H. (2022). In silico investigations of some Cyperus rotundus compounds as potential anti-inflammatory inhibitors of 5-LO and LTA4H enzymes. Journal of Biomolecular Structure and Dynamics, 40(22), 11571-11586.

Siddiqi, O. K., Ghebremichael, M., Musukuma, K., Dang, X., Mubanga, E., Birbeck, G. L., ... & Koralnik, I. J. (2021). LTA4H prevalence and mortality in adult Zambians with tuberculous meningitis. Annals of neurology, 90(6), 994-998.

Qin, R., Wang, H., & Yan, A. (2021). Classification and QSAR models of leukotriene A4 hydrolase (LTA4H) inhibitors by machine learning methods. SAR and QSAR in Environmental Research, 32(5), 411-431.

Maroteau, C., Espuela-Ortiz, A., Herrera-Luis, E., Srinivasan, S., Carr, F., Tavendale, R., ... & PiCA Consortium. (2021). LTA4H rs2660845 association with montelukast response in early and late-onset asthma. Plos one, 16(9), e0257396.

Wang, H., Xue, K., Duan, Z., Yang, Y., He, Z., Wu, C., ... & Tang, B. (2019). A photoacoustic and fluorescence dual-mode probe for LTA4H imaging reveals inflammation site in murine. Sensors and Actuators B: Chemical, 286, 243-249.

Zhao, S., Yao, K., Li, D., Liu, K., Jin, G., Yan, M., ... & Dong, Z. (2019). Inhibition of LTA4H by bestatin in human and mouse colorectal cancer. EBioMedicine, 44, 361-374.

Gao, W., An, C., Xue, X., Zheng, X., Niu, M., Zhang, Y., ... & Wang, B. (2019). Mass spectrometric analysis identifies AIMP1 and LTA4H as FSCN1‐binding proteins in laryngeal squamous cell carcinoma. Proteomics, 19(21-22), 1900059.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant LTA4H protein, His Tag (CAT#: V2LY-0526-LY8615)

Mouse Lta4h ELISA Kit (CAT#: V2LY-0626-LY1791)

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Isotype control

For research use only. Not intended for any clinical use.

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